| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87041-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | LMAB11048-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB19683-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB19684-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB19682-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75189-0.025mg | |||
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody [NBP1-87041] - Staining of human spleen shows strong cytoplasmic positivity in cells in white pulp.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-Immunohistochemistry-Paraffin-NBP1-87041-img0005.jpg)
![Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody [NBP1-87041] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-Immunohistochemistry-Paraffin-NBP1-87041-img0004.jpg)
![Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody [NBP1-87041] - Staining of human lymphoid tissues shows moderate cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-Immunohistochemistry-Paraffin-NBP1-87041-img0002.jpg)
![Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody [NBP1-87041] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-Immunohistochemistry-Paraffin-NBP1-87041-img0003.jpg)
![N/A Siglec-2/CD22 [Biotin]](http://images.novusbio.com/fullsize2/DATA_Siglec2_DY196805_ELISA_2383.jpg "N/A Siglec-2/CD22 [Biotin]")
![Flow Cytometry: Siglec-2/CD22 Antibody (hL22 (Epratuzumab)) - Chimeric [NBP2-75189] - Flow-cytometry using anti-CD22 antibody Epratuzumab. Rhesus monkey lymphocytes were stained with an isotype control (NBP2-52636, panel A) or the rabbit-chimeric version of Epratuzumab (NBP2-75189, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-hL22-Epratuzumab-Chimeric-Flow-Cytometry-NBP2-75189-img0003.jpg "Flow Cytometry: Siglec-2/CD22 Antibody (hL22 (Epratuzumab)) - Chimeric [NBP2-75189] - Flow-cytometry using anti-CD22 antibody Epratuzumab. Rhesus monkey lymphocytes were stained with an isotype control (NBP2-52636, panel A) or the rabbit-chimeric version of Epratuzumab (NBP2-75189, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Flow Cytometry: Siglec-2/CD22 Antibody (hL22 (Epratuzumab)) - Chimeric [NBP2-75189] - Flow-cytometry using anti-CD22 antibody Epratuzumab. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Eptratuzumab (NBP2-75189, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-hL22-Epratuzumab-Chimeric-Flow-Cytometry-NBP2-75189-img0001.jpg)
![Flow Cytometry: Siglec-2/CD22 Antibody (hL22 (Epratuzumab)) - Chimeric [NBP2-75189] - Flow-cytometry using anti-CD22 antibody Epratuzumab. Cynomolgus monkey lymphocytes were stained with an isotype control (NBP2-52636, panel A) or the rabbit-chimeric version of Epratuzumab (NBP2-75189, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-hL22-Epratuzumab-Chimeric-Flow-Cytometry-NBP2-75189-img0002.jpg)
![Flow Cytometry: Siglec-2/CD22 Antibody (RFB4) [NBP2-49890] - Flow Cytometry using Siglec-2/CD22 Antibody (RFB4).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-RFB4-Flow-Cytometry-NBP2-49890-img0001.jpg "Flow Cytometry: Siglec-2/CD22 Antibody (RFB4) [NBP2-49890] - Flow Cytometry using Siglec-2/CD22 Antibody (RFB4).")
![Western Blot: Siglec-2/CD22 Antibody (PO00-31) [NBP3-32136] - Western blot analysis of Siglec-2/CD22 on Daudi cell lysates with Rabbit anti-Siglec-2/CD22 antibody (NBP3-32136) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 95 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32136) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32136_rabbit-siglec-2-cd22-mab-po00-31-278202416331259.jpg "Western Blot: Siglec-2/CD22 Antibody (PO00-31) [NBP3-32136] - Western blot analysis of Siglec-2/CD22 on Daudi cell lysates with Rabbit anti-Siglec-2/CD22 antibody (NBP3-32136) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 95 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32136) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Siglec-2/CD22 Antibody (PO00-31) [NBP3-32136] - Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue with Rabbit anti-Siglec-2/CD22 antibody (NBP3-32136) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32136) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32136_rabbit-siglec-2-cd22-mab-po00-31-298202414115429.jpg)
![Flow Cytometry: Siglec-2/CD22 Antibody (S-HCL-1) [NBP3-09167] - Surface staining pattern of human peripheral blood stained using anti-human CD22 (S-HCL-1) purified antibody (concentration in sample 3 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-S-HCL-1-Flow-Cytometry-NBP3-09167-img0001.jpg "Flow Cytometry: Siglec-2/CD22 Antibody (S-HCL-1) [NBP3-09167] - Surface staining pattern of human peripheral blood stained using anti-human CD22 (S-HCL-1) purified antibody (concentration in sample 3 ug/ml) GAM APC.")
![Flow Cytometry: Siglec-2/CD22 Antibody (S-HCL-1) [NBP3-09167] - Separation of human CD22 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD22 (S-HCL-1) purified antibody (concentration in sample 3 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-S-HCL-1-Flow-Cytometry-NBP3-09167-img0002.jpg)
![Western Blot: Mouse Monoclonal Siglec-2/CD22 Antibody (S-HCL-1) [NBP3-09167] - The reactivity of S-HCL-1 antibody was assessed by comparing binding signals in a panel of three human B cell and one T cell line. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Raji, Ramos, Daudi, and Jurkat cell lines, mixed and heated (100C, 5 min) without reducing agent in SDS-loading buffer. Samples were resolved using 7% SDS-PAGE gel.Nitrocellulose membrane blot was probed with mouse IgG2b monoclonal antibody S-HCL-1 (2 ug/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green).CD22 was detected at ~110-125 kDa in Daudi cell line.](http://images.novusbio.com/fullsize/antibody/nbp3-09167_mouse-monoclonal-siglec-2-cd22-antibody-s-hcl-1-western-blot-11122024122650..jpg)