| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87041-25ul | |||
1968-SL), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line) as measured by the psuedoperoxidase assay. Adhesion elicited by Recombinant Human Siglec-2/CD22 Fc Chimera (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Siglec-2/CD22 Monoclonal Antibody (Catalog # MAB19682). The ND50 is typically 6-60 ng/mL." alt="Recombinant Human Siglec-2/CD22 Fc Chimera (Catalog # 1968-SL), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line) as measured by the psuedoperoxidase assay. Adhesion elicited by Recombinant Human Siglec-2/CD22 Fc Chimera (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Siglec-2/CD22 Monoclonal Antibody (Catalog # MAB19682). The ND50 is typically 6-60 ng/mL." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB19682-SP | |||
MAB19684) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Siglec-2/CD22 Monoclonal Antibody (Catalog # MAB19683) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human Siglec-2/CD22 protein was serially diluted 2-fold and captured by Mouse Anti-Human Siglec-2/CD22 Monoclonal Antibody ( Catalog # MAB19684) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Siglec-2/CD22 Monoclonal Antibody (Catalog # MAB19683) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB19683-SP | |||
DY990). Mouse Anti-Human Siglec-2/CD22 Monoclonal Antibody (Catalog # MAB19683) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human Siglec-2/CD22 protein was serially diluted 2-fold and captured by Mouse Anti-Human Siglec-2/CD22 Monoclonal Antibody (Catalog # MAB19684) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Siglec-2/CD22 Monoclonal Antibody (Catalog # MAB19683) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB19684-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | LMAB11048-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75189-0.025mg | |||
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody [NBP1-87041] - Staining of human spleen shows strong cytoplasmic positivity in cells in white pulp.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-Immunohistochemistry-Paraffin-NBP1-87041-img0005.jpg)
![Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody [NBP1-87041] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-Immunohistochemistry-Paraffin-NBP1-87041-img0004.jpg)
![Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody [NBP1-87041] - Staining of human lymphoid tissues shows moderate cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-Immunohistochemistry-Paraffin-NBP1-87041-img0002.jpg)
![Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody [NBP1-87041] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-Immunohistochemistry-Paraffin-NBP1-87041-img0003.jpg)
![N/A Siglec-2/CD22 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Siglec2_DY196805_ELISA_2383.jpg "N/A Siglec-2/CD22 [Biotin]")
![Flow Cytometry: Siglec-2/CD22 Antibody (RFB4) [NBP2-49890] - Flow Cytometry using Siglec-2/CD22 Antibody (RFB4).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-RFB4-Flow-Cytometry-NBP2-49890-img0001.jpg "Flow Cytometry: Siglec-2/CD22 Antibody (RFB4) [NBP2-49890] - Flow Cytometry using Siglec-2/CD22 Antibody (RFB4).")
![Flow Cytometry: Siglec-2/CD22 Antibody (hL22 (Epratuzumab)) - Chimeric [NBP2-75189] - Flow-cytometry using anti-CD22 antibody Epratuzumab. Rhesus monkey lymphocytes were stained with an isotype control (NBP2-52636, panel A) or the rabbit-chimeric version of Epratuzumab (NBP2-75189, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-hL22-Epratuzumab-Chimeric-Flow-Cytometry-NBP2-75189-img0003.jpg "Flow Cytometry: Siglec-2/CD22 Antibody (hL22 (Epratuzumab)) - Chimeric [NBP2-75189] - Flow-cytometry using anti-CD22 antibody Epratuzumab. Rhesus monkey lymphocytes were stained with an isotype control (NBP2-52636, panel A) or the rabbit-chimeric version of Epratuzumab (NBP2-75189, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Flow Cytometry: Siglec-2/CD22 Antibody (hL22 (Epratuzumab)) - Chimeric [NBP2-75189] - Flow-cytometry using anti-CD22 antibody Epratuzumab. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Eptratuzumab (NBP2-75189, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-hL22-Epratuzumab-Chimeric-Flow-Cytometry-NBP2-75189-img0001.jpg)
![Flow Cytometry: Siglec-2/CD22 Antibody (hL22 (Epratuzumab)) - Chimeric [NBP2-75189] - Flow-cytometry using anti-CD22 antibody Epratuzumab. Cynomolgus monkey lymphocytes were stained with an isotype control (NBP2-52636, panel A) or the rabbit-chimeric version of Epratuzumab (NBP2-75189, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-hL22-Epratuzumab-Chimeric-Flow-Cytometry-NBP2-75189-img0002.jpg)
![Immunohistochemistry: Siglec-2/CD22 Antibody (PO00-31) [NBP3-32136] - Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue with Rabbit anti-Siglec-2/CD22 antibody (NBP3-32136) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32136) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32136_rabbit-siglec-2-cd22-mab-po00-31-298202414115429.jpg "Immunohistochemistry: Siglec-2/CD22 Antibody (PO00-31) [NBP3-32136] - Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue with Rabbit anti-Siglec-2/CD22 antibody (NBP3-32136) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32136) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western blot analysis of Daudi cell lysates with NBP3-32136 at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 95 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32136 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32136_-western-blot-639204594550197035.jpg)
