| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-81124SS | |||
| BSA Free | NBP2-81124 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-81124SS | |||
| BSA Free | NBP2-81124 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-66379SS | |||
| BSA Free | NBP2-66379 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-66379SS | |||
| BSA Free | NBP2-66379 | |||
![Western Blot: CRISPR-Cas9 Antibody (JM11-55) [NBP2-67000] - Analysis of CRISPR-Cas9 SP on 293T transfected with Cas9 cell lysates with Rabbit anti-CRISPR-Cas9 SP antibody at 1/1,000 dilution.Lysates/proteins at 20 ug/Lane.Predicted band size: 158 kDaObserved band size: 158 kDaExposure time: 25 seconds; ECL: K1801;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67000_rabbit-monoclonal-crispr-cas9-antibody-jm11-55-western-blot-317202491743..jpg "Western Blot: CRISPR-Cas9 Antibody (JM11-55) [NBP2-67000] - Analysis of CRISPR-Cas9 SP on 293T transfected with Cas9 cell lysates with Rabbit anti-CRISPR-Cas9 SP antibody at 1/1,000 dilution.Lysates/proteins at 20 ug/Lane.Predicted band size: 158 kDaObserved band size: 158 kDaExposure time: 25 seconds; ECL: K1801;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: CRISPR-Cas9 Antibody [NBP2-52718] - Lane 1: 293T non transfected cell lysate. Lane 2: 293T Cas9 over expressed cell lysate. Load: 15ug per lane. Primary Antibody cas9 used at 1ug/mL overnight at 4C. Secondary Antibody: goat anti-rabbit HRP at 1:40,000 for 30 min at room temp. Expected theoretical molecular weight: 158 kDa.](http://images.novusbio.com/fullsize/CRISPR-Cas9-Antibody-Western-Blot-NBP2-52718-img0001.jpg "Western Blot: CRISPR-Cas9 Antibody [NBP2-52718] - Lane 1: 293T non transfected cell lysate. Lane 2: 293T Cas9 over expressed cell lysate. Load: 15ug per lane. Primary Antibody cas9 used at 1ug/mL overnight at 4C. Secondary Antibody: goat anti-rabbit HRP at 1:40,000 for 30 min at room temp. Expected theoretical molecular weight: 158 kDa.")
![Immunofluorescence: CRISPR-Cas9 Antibody [NBP2-52718] - HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1ug/mL. Secondary: Donkey anti-Rabbit at 1ug/mL, staining seen in red. Bottom images show GFP stain overlay](http://images.novusbio.com/fullsize/CRISPR-Cas9-Antibody-Immunofluorescence-NBP2-52718-img0002.jpg)