NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="Complement Component C3a was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human Complement Component C3a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3677) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3677-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85492-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85493-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 9620-C3-025/CF | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26471-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-23497-0.025ml | |||
![Western Blot: Complement C3 Antibody (5F9) [H00000718-M01] - C3 monoclonal antibody (M01), clone 5F9 Analysis of C3 expression in A-431.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-5F9-Western-Blot-H00000718-M01-img0007.jpg "Western Blot: Complement C3 Antibody (5F9) [H00000718-M01] - C3 monoclonal antibody (M01), clone 5F9 Analysis of C3 expression in A-431.")
![Immunohistochemistry-Paraffin: Complement C3 Antibody (5F9) [H00000718-M01] - Analysis of monoclonal antibody to C3 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration at 3 ug/mL.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-5F9-Immunohistochemistry-Paraffin-H00000718-M01-img0005.jpg)
![ELISA: Complement C3 Antibody (5F9) [H00000718-M01] - Detection limit for recombinant GST tagged C3 is approximately 1 ng/mL as a capture antibody.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-5F9-ELISA-H00000718-M01-img0008.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85492] - Staining of human adipose tissue, kidney, lung and testis using Anti-Complement C3 antibody NBP1-85492 (A) shows similar protein distribution across tissues to independent antibody NBP1-85493 (B).](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-85492-img0007.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85492] - Staining of human adipose tissue, kidney, lung and testis using Anti-Complement C3 antibody NBP1-85492 (A) shows similar protein distribution across tissues to independent antibody NBP1-85493 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85492] - Staining of human adipose tissue shows moderate extracellular space positivity in adipocytes.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-85492-img0001.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85492] - Staining of human lung shows strong extracellular space positivity in macrophages.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-85492-img0002.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85492] - Staining of human testis shows strong extracellular space positivity in Leydig cells.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-85492-img0003.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85492] - Staining of human kidney shows strong extracellular space positivity in cells in tubules.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-85492-img0004.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85493] - Staining of human adipose tissue, kidney, lung and testis using Anti-Complement C3 antibody NBP1-85493 (A) shows similar protein distribution across tissues to independent antibody NBP1-85492 (B).](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-85493-img0006.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85493] - Staining of human adipose tissue, kidney, lung and testis using Anti-Complement C3 antibody NBP1-85493 (A) shows similar protein distribution across tissues to independent antibody NBP1-85492 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85493] - Staining of human testis shows strong extracellular space positivity in Leydig cells.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-85493-img0009.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85493] - Staining of human adipose tissue shows strong extracellular space positivity in adipocytes.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-85493-img0004.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85493] - Staining of human kidney shows strong extracellular space positivity in cells in tubules.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-85493-img0007.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85493] - Staining of human lung shows strong extracellular space positivity in macrophages.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-85493-img0008.jpg)
![Immunohistochemistry-Paraffin: Complement C3d Antibody (E28-P) [NBP1-79074] - Formalin-fixed and paraffin-embedded human skin biopsy (4 um) from lesion of the early pemphigus vulgaris (without blister formation), stained with anti-C3d complement antibody shows strong positive intraepidermal intercellular immunostaining.](http://images.novusbio.com/fullsize/Complement-C3d-Antibody-E28-P-Immunohistochemistry-Paraffin-NBP1-79074-img0002.jpg "Immunohistochemistry-Paraffin: Complement C3d Antibody (E28-P) [NBP1-79074] - Formalin-fixed and paraffin-embedded human skin biopsy (4 um) from lesion of the early pemphigus vulgaris (without blister formation), stained with anti-C3d complement antibody shows strong positive intraepidermal intercellular immunostaining.")
![Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (HL1643) - Azide and BSA Free [NBP3-25389] - C3 antibody [HL1643] detects C3 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C3 stained by C3 antibody [HL1643] (NBP3-25389) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25389_rabbit-complement-c3-mab-hl1643-azide-and-bsa-free-2120249422664.jpg "Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (HL1643) - Azide and BSA Free [NBP3-25389] - C3 antibody [HL1643] detects C3 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C3 stained by C3 antibody [HL1643] (NBP3-25389) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Western Blot: Complement C3 Antibody (HL1643) - Azide and BSA Free [NBP3-25389] - Human plasma (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C3 antibody [HL1643] (NBP3-25389) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25389_rabbit-complement-c3-mab-hl1643-azide-and-bsa-free-2120249435517.jpg)
![Western Blot: Complement C3 Antibody (HL1643) - Azide and BSA Free [NBP3-25389] - Rat plasma (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C3 antibody [HL1643] (NBP3-25389) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25389_rabbit-complement-c3-mab-hl1643-azide-and-bsa-free-2120249362747.jpg)
![Immunohistochemistry: Complement C3 Antibody (4D12) [NBP3-26471] - Image of Complement C3 Antibody (4D12) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26471_rabbit-complement-c3-mab-4d12-262202415475630.jpg "Immunohistochemistry: Complement C3 Antibody (4D12) [NBP3-26471] - Image of Complement C3 Antibody (4D12) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: Complement C3 Antibody (4D12) [NBP3-26471] - Positive Western Blot detected in: THP-1 whole cell lysate, K562 whole cell lysate.<br /> All lanes: Complement C3 Antibody at 1: 1000<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 188 kDa<br /> Observed band size: 115 kDa<br />](http://images.novusbio.com/fullsize/nbp3-26471_rabbit-complement-c3-mab-4d12-28220241140917.jpg)
![Flow Cytometry: Complement C3 Antibody (4D12) [NBP3-26471] - Overlay histogram showing HepG2 cells stained with Complement C3 Antibody (4D12) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26471_rabbit-complement-c3-mab-4d12-282202414183687.jpg)
![ELISA: Monkey Complement C3 ELISA Kit (Colorimetric) [NBP3-18046] - Samples were spiked with high concentrations of Monkey Complement C3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-18046_monkey-complement-c3-elisa-kit-colorimetric-132202416225254.jpg "ELISA: Monkey Complement C3 ELISA Kit (Colorimetric) [NBP3-18046] - Samples were spiked with high concentrations of Monkey Complement C3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Monkey Complement C3 ELISA Kit (Colorimetric) [NBP3-18046] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-18046_monkey-complement-c3-elisa-kit-colorimetric-432024924022.jpg)
![Sandwich ELISA: Complement C3a Antibody (D17/1) - BSA Free [NBP2-23497] - Analysis using the Biotin conjugate of NBP2-23497. The calibration curve of a sandwich assay for Complement C3a using NBP1-05122 as the capture antibody and NBP2-23496 as the biotinylated detection antibody.](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-D17-1-BSA-Free-Sandwich-ELISA-NBP2-23497-img0002.jpg "Sandwich ELISA: Complement C3a Antibody (D17/1) - BSA Free [NBP2-23497] - Analysis using the Biotin conjugate of NBP2-23497. The calibration curve of a sandwich assay for Complement C3a using NBP1-05122 as the capture antibody and NBP2-23496 as the biotinylated detection antibody.")
