DY990). Goat Anti-Mouse TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog #
AF-410-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." class="big_lightbox">

DY990). Goat Anti-Mouse TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog #
AF-410-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." alt="Recombinant Mouse TNF-a protein was serially diluted 2-fold and captured by Rat Anti-Mouse TNF-a Monoclonal Antibody (Catalog # MAB4102) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Mouse TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog #
AF-410-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." class="big_thumb" />