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![Western Blot: Ubiquitin Antibody (3E2-E6) [H00006233-M01] - RPS27A monoclonal antibody (M01), clone 3E2-E6 Analysis of RPS27A expression in HL-60.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-3E2-E6-Western-Blot-H00006233-M01-img0002.jpg "Western Blot: Ubiquitin Antibody (3E2-E6) [H00006233-M01] - RPS27A monoclonal antibody (M01), clone 3E2-E6 Analysis of RPS27A expression in HL-60.")
![Western Blot: Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage [NBP2-36523] - Poly-Ubiquitin K63 Linkage Antibody (HWA4C4) [NBP2-36523] - Analysis of HeLa S100 Fraction. (Lane A) and MAb to Polyubiquitinylated Conjugates (clone FK1) (Lane B) at 1:1000 dilution.](http://images.novusbio.com/fullsize/Poly-Ubiquitin-K63-Linkage-Antibody-HWA4C4-Western-Blot-NBP2-36523-img0019.jpg "Western Blot: Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage [NBP2-36523] - Poly-Ubiquitin K63 Linkage Antibody (HWA4C4) [NBP2-36523] - Analysis of HeLa S100 Fraction. (Lane A) and MAb to Polyubiquitinylated Conjugates (clone FK1) (Lane B) at 1:1000 dilution.")
![Immunohistochemistry-Paraffin: Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage [NBP2-36523] - Poly-Ubiquitin K63 Linkage Antibody (HWA4C4) [NBP2-36523] - Sections of hippocampus from human Alzheimers disease brain stained at 1:100 dilution with biotinylated secondary antibody and DAB detection.](http://images.novusbio.com/fullsize/Poly-Ubiquitin-K63-Linkage-Antibody-HWA4C4-Immunohistochemistry-Paraffin-NBP2-36523-img0017.jpg)
![Western Blot: Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage [NBP2-36523] - Poly-Ubiquitin K63 Linkage Antibody (HWA4C4) [NBP2-36523] - Analysis at 1:1000 dilution](http://images.novusbio.com/fullsize/Poly-Ubiquitin-K63-Linkage-Antibody-HWA4C4-Western-Blot-NBP2-36523-img0018.jpg)
![Western Blot: Poly-Ubiquitin Antibody - M1 Linkage [NBP3-05631] - Western blot analysis of extracts of various cell lines, using Poly-Ubiquitin antibody (NBP3-05631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Poly-Ubiquitin-Antibody---M1-Linkage-Western-Blot-NBP3-05631-img0001.jpg "Western Blot: Poly-Ubiquitin Antibody - M1 Linkage [NBP3-05631] - Western blot analysis of extracts of various cell lines, using Poly-Ubiquitin antibody (NBP3-05631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Dot Blot: Poly-Ubiquitin Antibody - M1 Linkage [NBP3-05631] - Dot-blot analysis of all sorts of peptides using Poly-Ubiquitin antibody (NBP3-05631) at 1:1000 dilution.](http://images.novusbio.com/fullsize/Poly-Ubiquitin-Antibody---M1-Linkage-Dot-Blot-NBP3-05631-img0002.jpg)
![Dot Blot: Poly-Ubiquitin Antibody - M1 Linkage - Azide and BSA Free [Poly-Ubiquitin] - Dot-blot analysis of all sorts of peptides using Poly-Ubiquitin antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/nbp3-05631_rabbit-polyclonal-poly-ubiquitin-antibody-m1-linkage-5220251640268.jpg)
![Western Blot: Poly-Ubiquitin Antibody (5Y8J0) - K48 Linkage [NBP3-16232] - Western blot analysis of lysates from HeLa cells using Poly-Ubiquitin Rabbit mAb at 1:1000 dilution incubated overnight at 4C. HeLa cells were treated by MG132 (50 uM) at 37C for 90 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-16232_rabbit-poly-ubiquitin-mab-5y8j0-k48-linkage-5220251242554.jpg "Western Blot: Poly-Ubiquitin Antibody (5Y8J0) - K48 Linkage [NBP3-16232] - Western blot analysis of lysates from HeLa cells using Poly-Ubiquitin Rabbit mAb at 1:1000 dilution incubated overnight at 4C. HeLa cells were treated by MG132 (50 uM) at 37C for 90 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Immunocytochemistry/ Immunofluorescence: Poly-Ubiquitin Antibody (5Y8J0) - K48 Linkage [NBP3-16232] - Confocal imaging of MCF7 cells using Poly-Ubiquitin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16232_rabbit-poly-ubiquitin-mab-5y8j0-k48-linkage-5220251242553.jpg)
![Western Blot: Poly-Ubiquitin Antibody - K6 Linkage [NBP3-05680] - Western blot analysis of extracts of various cell lines, using Poly-Ubiquitin Rabbit pAb (NBP3-05680) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Poly-Ubiquitin-Antibody---K6-Linkage-Western-Blot-NBP3-05680-img0001.jpg "Western Blot: Poly-Ubiquitin Antibody - K6 Linkage [NBP3-05680] - Western blot analysis of extracts of various cell lines, using Poly-Ubiquitin Rabbit pAb (NBP3-05680) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Dot Blot: Poly-Ubiquitin Antibody - K6 Linkage [NBP3-05680] - Dot-blot analysis of all sorts of peptides using Poly-Ubiquitin antibody (NBP3-05680) at 1:1000 dilution.](http://images.novusbio.com/fullsize/Poly-Ubiquitin-Antibody---K6-Linkage-Dot-Blot-NBP3-05680-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Ubiquitin Antibody (FK1) [NBP3-11039] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone FK1. Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-FK1-Immunocytochemistry-Immunofluorescence-NBP3-11039-img0001.jpg "Immunocytochemistry/Immunofluorescence: Ubiquitin Antibody (FK1) [NBP3-11039] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone FK1. Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X.")
![Western Blot: Poly-Ubiquitin Antibody [NBP3-05664] - Western blot analysis of extracts of various cell lines, using Poly-Ubiquitin Rabbit pAb (NBP3-05664) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Poly-Ubiquitin-Antibody-Western-Blot-NBP3-05664-img0001.jpg "Western Blot: Poly-Ubiquitin Antibody [NBP3-05664] - Western blot analysis of extracts of various cell lines, using Poly-Ubiquitin Rabbit pAb (NBP3-05664) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.")
![Dot Blot: Poly-Ubiquitin Antibody - K29 Linkage - Azide and BSA Free [Poly-Ubiquitin] - Dot-blot analysis of all sorts of peptides using Poly-Ubiquitin antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/nbp3-05664_rabbit-polyclonal-poly-ubiquitin-antibody-52202516283269.jpg)
![Immunocytochemistry/Immunofluorescence: Ubiquitin Antibody [NBP2-58398] - Staining of human cell line MCF7 shows localization to nucleoli, cytosol & endoplasmic reticulum.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58398-img0001.jpg "Immunocytochemistry/Immunofluorescence: Ubiquitin Antibody [NBP2-58398] - Staining of human cell line MCF7 shows localization to nucleoli, cytosol & endoplasmic reticulum.")
![Western Blot: Ubiquitin Overexpression Lysate (Adult Normal) [NBL1-15568] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Ubiquitin.](http://images.novusbio.com/fullsize/Ubiquitin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15568-img0002.jpg "Western Blot: Ubiquitin Overexpression Lysate (Adult Normal) [NBL1-15568] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Ubiquitin.")
![Dot Blot: Poly-Ubiquitin Antibody - K63 Linkage [NBP3-05682] - Dot-blot analysis of all sorts of peptides using Poly-Ubiquitin antibody (NBP3-05682) at 1:1000 dilution.](http://images.novusbio.com/fullsize/Poly-Ubiquitin-Antibody---K63-Linkage-Dot-Blot-NBP3-05682-img0003.jpg "Dot Blot: Poly-Ubiquitin Antibody - K63 Linkage [NBP3-05682] - Dot-blot analysis of all sorts of peptides using Poly-Ubiquitin antibody (NBP3-05682) at 1:1000 dilution.")