We're moving to rndsystems.com. Come with us!
After August 17, 2026, Novus Biologicals products and services will no longer be available on this website; you will access all products and services on rndsystems.com. Create your R&D Systems online account today.
![ELISA: Mouse eNOS ELISA Kit (Colorimetric) [NBP2-80135] - Samples were spiked with high concentrations of Mouse eNOS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-80135_mouse-enos-elisa-kit-colorimetric-132202416175663.jpg "ELISA: Mouse eNOS ELISA Kit (Colorimetric) [NBP2-80135] - Samples were spiked with high concentrations of Mouse eNOS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse eNOS ELISA Kit (Colorimetric) [NBP2-80135] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-80135_mouse-enos-elisa-kit-colorimetric-4320248574382.jpg)
![Western Blot: eNOS Overexpression Lysate (Adult Normal) [NBP2-10685] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for eNOS.](http://images.novusbio.com/fullsize/eNOS-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10685-img0002.jpg "Western Blot: eNOS Overexpression Lysate (Adult Normal) [NBP2-10685] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for eNOS.")
![Western Blot: eNOS Antibody [NBP3-23180] - Western blot analysis of human umbilical vein endothelial cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3, 5 & 7) then the blots were treated with lambda phosphatase (lanes 2, 4, 6 & 8). The blots were probed with anti-endothelial nitric oxide synthase (eNOS) (C-terminal region) (lanes 1 & 2), anti-eNOS (Ser-632) (lanes 3 & 4), anti-eNOS (Ser-1177) (lanes 5 & 6) and anti-eNOS (a.a. 1172-1181) (lanes 7 & 8).](http://images.novusbio.com/fullsize/nbp3-23180_rabbit-enos-pab-19920231759442.jpg "<b>Biological Strategies Validation. </b>Western Blot: eNOS Antibody [NBP3-23180] - Western blot analysis of human umbilical vein endothelial cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3, 5 & 7) then the blots were treated with lambda phosphatase (lanes 2, 4, 6 & 8). The blots were probed with anti-endothelial nitric oxide synthase (eNOS) (C-terminal region) (lanes 1 & 2), anti-eNOS (Ser-632) (lanes 3 & 4), anti-eNOS (Ser-1177) (lanes 5 & 6) and anti-eNOS (a.a. 1172-1181) (lanes 7 & 8).")
![SDS-Page: Recombinant Human eNOS Protein [H00004846-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00004846-Q01-1.jpg "SDS-Page: Recombinant Human eNOS Protein [H00004846-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![ELISA: Rat eNOS ELISA Kit (Colorimetric) [NBP2-80136] - Samples were spiked with high concentrations of Rat eNOS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-80136_rat-enos-elisa-kit-colorimetric-132202416184841.jpg "ELISA: Rat eNOS ELISA Kit (Colorimetric) [NBP2-80136] - Samples were spiked with high concentrations of Rat eNOS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat eNOS ELISA Kit (Colorimetric) [NBP2-80136] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-80136_rat-enos-elisa-kit-colorimetric-4320249151626.jpg)
![Western Blot: eNOS [p Ser116] Antibody [NBP2-29664] - Untreated (lane 1) and lambda phosphatase-treated (lane 2) Fetal bovine heart endothelial cell lysate was probed with anti-phospho-eNOS (1:1000).](http://images.novusbio.com/fullsize/eNOS-[p-Ser116]-Antibody-Western-Blot-NBP2-29664-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: eNOS [p Ser116] Antibody [NBP2-29664] - Untreated (lane 1) and lambda phosphatase-treated (lane 2) Fetal bovine heart endothelial cell lysate was probed with anti-phospho-eNOS (1:1000).")