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![Sandwich ELISA: SARS-CoV-2 Nucleocapsid Antibody (NP642) [NBP3-07072] - Sandwich ELISA for SARS-CoV-2 Nucleocapsid Matched Pair Antibodies. Antibodies: SARS-CoV-2 Nucleocapsid Antibodies (NBP3-07072). A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (NBP3-07072, 5ug/ml) as capture antibody, a Nucleocapsid recombinant protein as the binding protein, and the anti-SARS-CoV-2 Nucleocapsid antibody (NBP3-07068, 1ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 2.5 ng](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-NP642-Sandwich-ELISA-NBP3-07072-img0001.jpg "Sandwich ELISA: SARS-CoV-2 Nucleocapsid Antibody (NP642) [NBP3-07072] - Sandwich ELISA for SARS-CoV-2 Nucleocapsid Matched Pair Antibodies. Antibodies: SARS-CoV-2 Nucleocapsid Antibodies (NBP3-07072). A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (NBP3-07072, 5ug/ml) as capture antibody, a Nucleocapsid recombinant protein as the binding protein, and the anti-SARS-CoV-2 Nucleocapsid antibody (NBP3-07068, 1ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 2.5 ng")
![Sandwich ELISA: SARS-CoV-2 Nucleocapsid Antibody (NP642) [NBP3-07072] - Sandwich ELISA for SARS-CoV-2 Nucleocapsid Matched Pair Antibodies. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (NBP3-07072, 5ug/ml) as capture antibody, a Nucleocapsid recombinant protein as the binding protein, and the anti-SARS-CoV-2 Nucleocapsid antibody (NBP3-07070, 1ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 2.7 ng](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-NP642-Sandwich-ELISA-NBP3-07072-img0003.jpg)
![Immunohistochemistry: SARS-CoV-2 Nucleocapsid Antibody (NP642) - Azide and BSA Free [NBP3-07072] - Validation of SARS-CoV-2 (COVID-19) Nucleocapsid in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Nucleocapsid antibody (1 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Nucleocapsid protein was observed in the macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.](http://images.novusbio.com/fullsize/nbp3-07072_rabbit-monoclonal-sars-cov-2-nucleocapsid-antibody-np642-2711202420312942.gif)
![Western Blot: SARS-CoV-2 Nucleocapsid Antibody [NBP3-07039] - Coomassie blue staining non-reduced and reduced SDS-PAGE analysis](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-Western-Blot-NBP3-07039-img0001.jpg "Western Blot: SARS-CoV-2 Nucleocapsid Antibody [NBP3-07039] - Coomassie blue staining non-reduced and reduced SDS-PAGE analysis")
![Sandwich ELISA: SARS-CoV-2 Nucleocapsid Antibody [NBP3-07039] - Sandwich ELISA for SARS-CoV-2 Nucleocapsid Matched Pair Antibodies. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (NBP3-07039, 2ug/ml) as capture antibody, a nucleocapsid recombinant protein as the binding protein, and the anti-SARS-CoV-2 Nucleocapsid antibody (NBP3-07068, 0.5ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 15.82 ng](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-Sandwich-ELISA-NBP3-07039-img0002.jpg)
![Western Blot: SARS-CoV-2 Nucleocapsid Antibody (N009) [NBP3-05721] - Western Blot Validation with Recombinant Protein. Loading: 1ug of SARS-CoV-2 (COVID-19) nucleocapsid recombinant protein per lane. Antibodies: SARS-CoV-2 Nucleocapsid Antibody, NBP3-05721, 1:2000. Secondary: Goat anti-human IgG HRP conjugate at 1:5000 dilution.](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-N009-Western-Blot-NBP3-05721-img0002.jpg "Western Blot: SARS-CoV-2 Nucleocapsid Antibody (N009) [NBP3-05721] - Western Blot Validation with Recombinant Protein. Loading: 1ug of SARS-CoV-2 (COVID-19) nucleocapsid recombinant protein per lane. Antibodies: SARS-CoV-2 Nucleocapsid Antibody, NBP3-05721, 1:2000. Secondary: Goat anti-human IgG HRP conjugate at 1:5000 dilution.")
![ELISA: SARS-CoV-2 Nucleocapsid Antibody (N009) [NBP3-05721] - ELISA Coating original concentration: 2 ug/mL, 100 uL/well sample is SARS-CoV-2 Nucleocapsid Recombinant Protein, 100 uL/well. Stop buffer 50 uL/well.](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-N009-ELISA-NBP3-05721-img0001.jpg)
![Sandwich ELISA: SARS-CoV-2 Nucleocapsid Antibody (3868) [NBP3-07070] - Sandwich ELISA for SARS-CoV-2 Nucleocapsid Matched Pair Antibodies. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (NBP3-07038, 2ug/ml) as capture antibody, a nucleocapsid recombinant protein as the binding protein, and the anti-SARS-CoV-2 Nucleocapsid antibody (NBP3-07070, 0.5ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 4.27 ng](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-3868-Sandwich-ELISA-NBP3-07070-img0001.jpg "Sandwich ELISA: SARS-CoV-2 Nucleocapsid Antibody (3868) [NBP3-07070] - Sandwich ELISA for SARS-CoV-2 Nucleocapsid Matched Pair Antibodies. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (NBP3-07038, 2ug/ml) as capture antibody, a nucleocapsid recombinant protein as the binding protein, and the anti-SARS-CoV-2 Nucleocapsid antibody (NBP3-07070, 0.5ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 4.27 ng")
![Sandwich ELISA: SARS-CoV-2 Nucleocapsid Antibody (3868) [NBP3-07070] - Sandwich ELISA for SARS-CoV-2 Nucleocapsid Antibodies. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (NBP3-07039, 2ug/ml) as capture antibody, the Nucleocapsid recombinant protein as the binding protein, and the anti-SARS-CoV-2 Nucleocapsid antibody (NBP3-07068, 0.5ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 15.82 ng](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-3868-Sandwich-ELISA-NBP3-07070-img0002.jpg)
![ELISA: SARS-CoV-2 Nucleocapsid Antibody (NP1189) [NBP3-07071] - Sandwich ELISA for SARS-CoV-2 Nucleocapsid Matched Pair Antibodies. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (NBP3-07069, 2 ug/mL) as capture antibody, a Nucleocapsid recombinant protein as the binding protein, and the anti-SARS-CoV-2 Nucleocapsid antibody (NBP3-07071, 0.1 ug/mL) as the detection antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 6.9 ng.](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-NP1189-Sandwich-ELISA-NBP3-07071-img0001.jpg "ELISA: SARS-CoV-2 Nucleocapsid Antibody (NP1189) [NBP3-07071] - Sandwich ELISA for SARS-CoV-2 Nucleocapsid Matched Pair Antibodies. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (NBP3-07069, 2 ug/mL) as capture antibody, a Nucleocapsid recombinant protein as the binding protein, and the anti-SARS-CoV-2 Nucleocapsid antibody (NBP3-07071, 0.1 ug/mL) as the detection antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 6.9 ng.")
![Immunohistochemistry: SARS-CoV-2 Nucleocapsid Antibody (NP1189) - Azide and BSA Free [NBP3-07071] - Validation of SARS-CoV-2 (COVID-19) Nucleocapsid in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Nucleocapsid antibody (1 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Nucleocapsid protein was observed in the macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.](http://images.novusbio.com/fullsize/nbp3-07071_rabbit-monoclonal-sars-cov-2-nucleocapsid-antibody-np1189-2711202420272423.jpg)
![ELISA: SARS-CoV-2 Nucleocapsid Antibody (75G5a) [NBP3-07040] - Microtiter wells were coated with SARS-CoV-2 Nucleocapsid and SARS-CoV Nucleocapsid at 1 ug/mL. Purified rabbit monoclonal antibodies were serially diluted 1:2 starting at 1 ug/mL, which showed very strong and specific binding to COVID-19 Nucleocapsid antigen, with no significant cross-reactivity to SARS-CoV Nucleocapsid antigen.](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-75G5a-ELISA-NBP3-07040-img0001.jpg "ELISA: SARS-CoV-2 Nucleocapsid Antibody (75G5a) [NBP3-07040] - Microtiter wells were coated with SARS-CoV-2 Nucleocapsid and SARS-CoV Nucleocapsid at 1 ug/mL. Purified rabbit monoclonal antibodies were serially diluted 1:2 starting at 1 ug/mL, which showed very strong and specific binding to COVID-19 Nucleocapsid antigen, with no significant cross-reactivity to SARS-CoV Nucleocapsid antigen.")
![Sandwich ELISA: SARS-CoV-2 Nucleocapsid Antibody (75G5a) [NBP3-07040] - A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid specific rabbit monoclonal antibodies: NBP3-07040 as a capture antibody and a biotin-conjugated antibody as a detection antibody. SARS-CoV-2 Nucleocapsid was serially diluted 1:2 starting at 25 ng/mL. Rabbit monoclonal antibodies NBP3-07040 and a biotin-conjugated antibody detected SARS-CoV-2 Nucleocapsid antigen at very high sensitivity as low as 100 pg/mL (100 pg).](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-75G5a-Sandwich-ELISA-NBP3-07040-img0002.jpg)
![Sandwich ELISA: SARS-CoV-2 Nucleocapsid Antibody (75G5a) [NBP3-07040] - A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid specific rabbit monoclonal antibodies: NBP3-07040 as a capture antibody and a biotin-conjugated antibody as a detection antibody. SARS-CoV-2 Nucleocapsid was serially diluted 1:2 starting at 25 ng/mL. Rabbit monoclonal antibodies NBP3-07040 and a biotin-conjugated antibody detected SARS-CoV-2 Nucleocapsid antigen at very high sensitivity as low as 25 pg/mL (25 pg).](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-75G5a-Sandwich-ELISA-NBP3-07040-img0003.jpg)
![ELISA: SARS-CoV-2 Nucleocapsid Antibody (ARC5077-02) - Azide and BSA Free [NBP3-05765] - No cross-reactivity in ELISA with MERS-CoV Nucleoprotein protein and SARS Coronavirus Nucleocapsid.](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-ARC5077-02-Azide-and-BSA-Free-ELISA-NBP3-05765-img0002.jpg "ELISA: SARS-CoV-2 Nucleocapsid Antibody (ARC5077-02) - Azide and BSA Free [NBP3-05765] - No cross-reactivity in ELISA with MERS-CoV Nucleoprotein protein and SARS Coronavirus Nucleocapsid.")
![ELISA: SARS-CoV-2 Nucleocapsid Antibody (ARC5077-02) - Azide and BSA Free [NBP3-05765] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-ARC5077-02-Azide-and-BSA-Free-ELISA-NBP3-05765-img0001.jpg)
![Western Blot: SARS-CoV-2 Nucleocapsid Antibody (019) [NBP3-12735] - Lane A: SARS-COV-2 Nucleocapsid overexpressed HEK293 Whole Cell LysateLane B: HEK293 Whole Cell LysateLysates/proteins at 10 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing condition](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-019-Western-Blot-NBP3-12735-img0003.jpg "Western Blot: SARS-CoV-2 Nucleocapsid Antibody (019) [NBP3-12735] - Lane A: SARS-COV-2 Nucleocapsid overexpressed HEK293 Whole Cell LysateLane B: HEK293 Whole Cell LysateLysates/proteins at 10 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing condition")
![Western Blot: SARS-CoV-2 Nucleocapsid Antibody (6A8) [NBP3-05729] - Validation of SARS-CoV-2 Nucleocapsid AntibodyLane 1: 50ng, Lane 2: 25ng, Lane 3: 5ng, Lane 4: 2ng , Lane 5: 1ng and Lane 6: 0.5ng of SARS-CoV-2 N protein probed with SARS-CoV-2 Nucleocapsid Antibody [6A8], at 1:1000 dilution and 4C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37C.](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-6A8-Western-Blot-NBP3-05729-img0001.jpg "Western Blot: SARS-CoV-2 Nucleocapsid Antibody (6A8) [NBP3-05729] - Validation of SARS-CoV-2 Nucleocapsid AntibodyLane 1: 50ng, Lane 2: 25ng, Lane 3: 5ng, Lane 4: 2ng , Lane 5: 1ng and Lane 6: 0.5ng of SARS-CoV-2 N protein probed with SARS-CoV-2 Nucleocapsid Antibody [6A8], at 1:1000 dilution and 4C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37C.")