| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52438-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89696-50ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| HRP | NBP2-79313-5Plates | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90396-50ul | |||
![N/A CD200/OX2 [Biotin]](http://images.novusbio.com/fullsize2/DATA_CD200_DY2724_ELISA_1879.jpg "N/A CD200/OX2 [Biotin]")
![Western Blot: CD200/OX2 Antibody (PSH01-49) [NBP3-32133] - Western blot analysis of CD200/OX2 on different lysates with Rabbit anti-CD200/OX2 antibody (NBP3-32133) at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate Lane 2: NCI-H226 cell lysate Lane 3: Human brain tissue lysate Lane 4: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 31 kDa Observed band size: 40-50kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32133) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32133_rabbit-cd200-ox2-mab-psh01-49-278202415212462.jpg "Western Blot: CD200/OX2 Antibody (PSH01-49) [NBP3-32133] - Western blot analysis of CD200/OX2 on different lysates with Rabbit anti-CD200/OX2 antibody (NBP3-32133) at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate Lane 2: NCI-H226 cell lysate Lane 3: Human brain tissue lysate Lane 4: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 31 kDa Observed band size: 40-50kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32133) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD200/OX2 Antibody (PSH01-49) [NBP3-32133] - Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-CD200/OX2 antibody (NBP3-32133) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32133) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32133_rabbit-cd200-ox2-mab-psh01-49-298202413585577.jpg)
![Flow Cytometry: CD200/OX2 Antibody (PSH01-49) [NBP3-32133] - Flow cytometric analysis of SK-MEL-28 cells labeling CD200/OX2. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32133, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32133_rabbit-cd200-ox2-mab-psh01-49-392024884632.jpg)
![Flow Cytometry: CD200/OX2 Antibody (OX-104) [NB100-64860] - Surface staining of CD200 in human peripheral blood with CD200/OX2 antibody (conjugated to FITC).](http://images.novusbio.com/fullsize/CD200-OX2-Antibody-OX-104-Flow-Cytometry-NB100-64860-img0003.jpg "Flow Cytometry: CD200/OX2 Antibody (OX-104) [NB100-64860] - Surface staining of CD200 in human peripheral blood with CD200/OX2 antibody (conjugated to FITC).")
![Flow Cytometry: CD200/OX2 Antibody (OX-104) [NB100-64860] - Surface staining of CD200 in human peripheral blood with CD200/OX2 antibody (conjugated to PE).](http://images.novusbio.com/fullsize/CD200-OX2-Antibody-OX-104-Flow-Cytometry-NB100-64860-img0001.jpg)
![Flow Cytometry: CD200/OX2 Antibody (OX-104) [NB100-64860] - Surface staining of CD200 in human peripheral blood with CD200/OX2 antibody (conjugated to APC).](http://images.novusbio.com/fullsize/CD200-OX2-Antibody-OX-104-Flow-Cytometry-NB100-64860-img0002.jpg)
![Flow Cytometry: CD200/OX2 Antibody (OX-2) [NB100-65320] - Analysis of PE conjugate of NB100-65320. Staining of rat thymocytes with Mouse anti Rat CD200](http://images.novusbio.com/fullsize/CD200-OX2-Antibody-OX-2-Flow-Cytometry-NB100-65320-img0002.jpg "Flow Cytometry: CD200/OX2 Antibody (OX-2) [NB100-65320] - Analysis of PE conjugate of NB100-65320. Staining of rat thymocytes with Mouse anti Rat CD200")
![Flow Cytometry: CD200/OX2 Antibody (OX-2) [NB100-65320] - Staining of rat thymocytes with Mouse anti Rat CD200/OX2.](http://images.novusbio.com/fullsize/CD200-OX2-Antibody-OX-2-Flow-Cytometry-NB100-65320-img0001.jpg)
![Western Blot: CD200/OX2 Antibody (6E8B11) [NBP2-52438] - 1: Western blot analysis using CD200 mAb against human CD200 (AA: Extra(56-257)) recombinant protein. (Expected MW is 48.4 kDa)](http://images.novusbio.com/fullsize/CD200-OX2-Antibody-6E8B11-Western-Blot-NBP2-52438-img0001.jpg "Western Blot: CD200/OX2 Antibody (6E8B11) [NBP2-52438] - 1: Western blot analysis using CD200 mAb against human CD200 (AA: Extra(56-257)) recombinant protein. (Expected MW is 48.4 kDa)")
![Western Blot: CD200/OX2 Antibody (6E8B11) [NBP2-52438] - Analysis using CD200 mAb against HEK293 (1) and CD200 (AA: Extra(56-257))-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CD200-OX2-Antibody-6E8B11-Western-Blot-NBP2-52438-img0002.jpg)
![ELISA: CD200/OX2 Antibody (6E8B11) [NBP2-52438] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/CD200-OX2-Antibody-6E8B11-ELISA-NBP2-52438-img0003.jpg)
![ELISA: Human CD200/OX2 ELISA Kit (Colorimetric) [NBP2-75134] - Samples were spiked with high concentrations of Human CD200/OX2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75134_human-cd200-ox2-elisa-kit-colorimetric-13220241620479.jpg "ELISA: Human CD200/OX2 ELISA Kit (Colorimetric) [NBP2-75134] - Samples were spiked with high concentrations of Human CD200/OX2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human CD200/OX2 ELISA Kit (Colorimetric) [NBP2-75134] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75134_human-cd200-ox2-elisa-kit-colorimetric-432024973637.jpg)
![Western Blot: CD200/OX2 Overexpression Lysate (Adult Normal) [NBP2-04304] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CD200/OX2.](http://images.novusbio.com/fullsize/CD200-OX2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04304-img0001.jpg "Western Blot: CD200/OX2 Overexpression Lysate (Adult Normal) [NBP2-04304] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CD200/OX2.")
![Immunohistochemistry-Paraffin: CD200/OX2 Antibody (333) [NBP2-89696] - Staining of human CD200 in human kidney with rabbit monoclonal antibody (1:100).](http://images.novusbio.com/fullsize/CD200-OX2-Antibody-333-Immunohistochemistry-Paraffin-NBP2-89696-img0002.jpg "Immunohistochemistry-Paraffin: CD200/OX2 Antibody (333) [NBP2-89696] - Staining of human CD200 in human kidney with rabbit monoclonal antibody (1:100).")
![Immunohistochemistry-Paraffin: CD200/OX2 Antibody (333) [NBP2-89696] - Staining of human CD200 in human tonsil with rabbit monoclonal antibody (1:100).](http://images.novusbio.com/fullsize/CD200-OX2-Antibody-333-Immunohistochemistry-Paraffin-NBP2-89696-img0001.jpg)
![Sandwich ELISA: CD200/OX2 Antibody Pair [HRP] [NBP2-79313] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.](http://images.novusbio.com/fullsize/CD200-OX2-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79313-img0001.jpg "Sandwich ELISA: CD200/OX2 Antibody Pair [HRP] [NBP2-79313] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.")
![Flow Cytometry: CD200/OX2 Antibody (002) [NBP2-90396] - Analysis of CD200 expression on spleen lymphocytes. BALB/c splenocytes were stained with Mouse CD200 Monoclonal Ab (50074-R002), then a FITC-conjugated second step antibody. The histogram were derived from the gated events based on light scattering characteristics of lymphocytes.](http://images.novusbio.com/fullsize/CD200-OX2-Antibody-002-Flow-Cytometry-NBP2-90396-img0001.jpg "Flow Cytometry: CD200/OX2 Antibody (002) [NBP2-90396] - Analysis of CD200 expression on spleen lymphocytes. BALB/c splenocytes were stained with Mouse CD200 Monoclonal Ab (50074-R002), then a FITC-conjugated second step antibody. The histogram were derived from the gated events based on light scattering characteristics of lymphocytes.")