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![Western Blot: Tau [p Thr205] Antibody - Azide and BSA Free [NBP3-16889] - Western blot analysis of lysates from Rat brain, using Tau Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-16889_rabbit-tau-p-thr-205-pab-52202511402761.jpg "Western Blot: Tau [p Thr205] Antibody - Azide and BSA Free [NBP3-16889] - Western blot analysis of lysates from Rat brain, using Tau Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.")
![Western Blot: Tau Antibody (S04-2G8) [NBP3-20060] - Western blot detection of Tau in Rat Brain lysates using NBP3-20060 (1:1000 diluted). Predicted band size: 79kDa. Observed band size: 50-80kDa.](http://images.novusbio.com/fullsize/Tau-Antibody-S04-2G8-Western-Blot-NBP3-20060-img0001.jpg "Western Blot: Tau Antibody (S04-2G8) [NBP3-20060] - Western blot detection of Tau in Rat Brain lysates using NBP3-20060 (1:1000 diluted). Predicted band size: 79kDa. Observed band size: 50-80kDa.")
![Untreated (–) and treated (+) SH-SY5Y whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with Tau (pSer235) antibody (HL3899) diluted at 1:1000 and Tau antibody [HL2982] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45162_rabbit-tau-p-ser235-mab-hl3899-azide-and-bsa-free-western-blot-2942025155710..jpg "<b>Biological Strategies Validation. </b>Untreated (–) and treated (+) SH-SY5Y whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with Tau (pSer235) antibody (HL3899) diluted at 1:1000 and Tau antibody [HL2982] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Tau Overexpression Lysate (Adult Normal) [NBP2-06994] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Tau.](http://images.novusbio.com/fullsize/Tau-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-06994-img0001.jpg "Western Blot: Tau Overexpression Lysate (Adult Normal) [NBP2-06994] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Tau.")
![Western Blot: Tau Overexpression Lysate (Adult Normal) [NBP2-11274] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Tau.](http://images.novusbio.com/fullsize/Tau-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11274-img0001.jpg "Western Blot: Tau Overexpression Lysate (Adult Normal) [NBP2-11274] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Tau.")
![Western Blot: Tau Overexpression Lysate (Adult Normal) [NBP2-11394] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Tau.](http://images.novusbio.com/fullsize/Tau-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11394-img0001.jpg "Western Blot: Tau Overexpression Lysate (Adult Normal) [NBP2-11394] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Tau.")
![Western Blot: Tau Overexpression Lysate (Adult Normal) [NBL1-12894] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Tau.](http://images.novusbio.com/fullsize/Tau-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12894-img0002.jpg "Western Blot: Tau Overexpression Lysate (Adult Normal) [NBL1-12894] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Tau.")
![Western Blot: Tau Overexpression Lysate (Adult Normal) [NBL1-12895] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Tau.](http://images.novusbio.com/fullsize/Tau-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12895-img0002.jpg "Western Blot: Tau Overexpression Lysate (Adult Normal) [NBL1-12895] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Tau.")
