MAB10498) coated on a Clear Polystyrene Microplate (
DY990). Mouse Anti-Human Indoleamine 2,3‑dioxygenase/IDO Monoclonal Antibody (Catalog # MAB104981) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (
DY998) followed by Substrate Solution (
DY999) and stopping the enzymatic reaction with Stop Solution (
DY994 )." class="big_lightbox">
MAB10498) coated on a Clear Polystyrene Microplate (
DY990). Mouse Anti-Human Indoleamine 2,3‑dioxygenase/IDO Monoclonal Antibody (Catalog # MAB104981) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (
DY998) followed by Substrate Solution (
DY999) and stopping the enzymatic reaction with Stop Solution (
DY994 )." alt="Recombinant Human Indoleamine 2,3‑dioxygenase/IDO protein was serially diluted 2-fold and captured by Mouse Anti-Human Indoleamine 2,3‑dioxygenase/IDO Monoclonal Antibody (
MAB10498) coated on a Clear Polystyrene Microplate (
DY990). Mouse Anti-Human Indoleamine 2,3‑dioxygenase/IDO Monoclonal Antibody (Catalog # MAB104981) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (
DY998) followed by Substrate Solution (
DY999) and stopping the enzymatic reaction with Stop Solution (
DY994 )." class="big_thumb" />
DY990). Mouse Anti-Human Indoleamine 2,3‑dioxygenase/IDO Monoclonal Antibody (MAB104981) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." alt="Recombinant Human Indoleamine 2,3‑dioxygenase/IDO protein was serially diluted 2-fold and captured by Mouse Anti-Human Indoleamine 2,3‑dioxygenase/IDO Monoclonal Antibody (Catalog # MAB10498) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human Indoleamine 2,3‑dioxygenase/IDO Monoclonal Antibody (MAB104981) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_thumb" />
MAB10498) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human Indoleamine 2,3‑dioxygenase/IDO Monoclonal Antibody (Catalog # MAB104981) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994 )." alt="Recombinant Human Indoleamine 2,3‑dioxygenase/IDO protein was serially diluted 2-fold and captured by Mouse Anti-Human Indoleamine 2,3‑dioxygenase/IDO Monoclonal Antibody (MAB10498) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human Indoleamine 2,3‑dioxygenase/IDO Monoclonal Antibody (Catalog # MAB104981) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994 )." class="big_thumb" />
![Immunohistochemistry: Indoleamine 2,3-dioxygenase/IDO Antibody (PD00-62) [NBP3-32457] - Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissue with Rabbit anti-Indoleamine 2,3-dioxygenase/IDO antibody (NBP3-32457) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32457) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32457_rabbit-indoleamine-2-3-dioxygenase-ido-mab-pd00-62-298202413585591.jpg "Immunohistochemistry: Indoleamine 2,3-dioxygenase/IDO Antibody (PD00-62) [NBP3-32457] - Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissue with Rabbit anti-Indoleamine 2,3-dioxygenase/IDO antibody (NBP3-32457) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32457) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Indoleamine 2,3-dioxygenase/IDO Antibody (PD00-62) [NBP3-32457] - Immunocytochemistry analysis of HeLa cells treated with or without 50 ng/mL IFN-gamma for 16 hours labeling Indoleamine 2,3-dioxygenase/IDO with Rabbit anti-Indoleamine 2,3-dioxygenase/IDO antibody (NBP3-32457) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Indoleamine 2,3-dioxygenase/IDO antibody (NBP3-32457) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32457_rabbit-indoleamine-2-3-dioxygenase-ido-mab-pd00-62-2920241192334.jpg)
![Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (BLR040F) [NBP2-76408] - Detection of human IDO1 by WB of HeLa treated with IFN-gamma (+) or mock treated (-). Antibody: Rabbit anti-IDO1 recombinant monoclonal [BLR040F] (NBP2-76408). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-Vinculin.](http://images.novusbio.com/fullsize/Indoleamine-2-3-dioxygenase-IDO-Antibody-BLR040F-Western-Blot-NBP2-76408-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (BLR040F) [NBP2-76408] - Detection of human IDO1 by WB of HeLa treated with IFN-gamma (+) or mock treated (-). Antibody: Rabbit anti-IDO1 recombinant monoclonal [BLR040F] (NBP2-76408). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-Vinculin.")
![<b>Biological Strategies Validation. </b>Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (BLR040F) [NBP2-76408] - Detection of human IDO1 by WB of immunoprecipitates from HeLa lysate treated with IFN-gamma. Antibodies: Rabbit anti-IDO1 recombinant monoclonal [BLR040F] (NBP2-76408) and rabbit anti-IDO1 recombinant monoclonal [BLR031F] (NBP2-76404).](http://images.novusbio.com/fullsize/Indoleamine-2-3-dioxygenase-IDO-Antibody-BLR040F-Western-Blot-NBP2-76408-img0001.jpg)
![Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (SR1771) [NBP3-21777] - Western blot analysis using NBP3-21777 in HeLa cell lysate treated with IFN gamma.](http://images.novusbio.com/fullsize/nbp3-21777_rabbit-indoleamine-2-3-dioxygenase-ido-mab-sr1771-5720231624728.jpg "<b>Biological Strategies Validation. </b>Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (SR1771) [NBP3-21777] - Western blot analysis using NBP3-21777 in HeLa cell lysate treated with IFN gamma.")
![Western Blot: IDO1 Overexpression Lysate (Adult Normal) [NBL1-11988] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IDO1.](http://images.novusbio.com/fullsize/IDO1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11988-img0002.jpg "Western Blot: IDO1 Overexpression Lysate (Adult Normal) [NBL1-11988] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IDO1.")
![SDS-Page: Recombinant Human Indoleamine 2,3-dioxygenase/IDO Protein [NBP2-51723] - 15% SDS Page (3 ug)](http://images.novusbio.com/fullsize/Recombinant-Human-Indoleamine-2-3-dioxygenase-IDO-Protein-SDS-Page-NBP2-51723-img0002.jpg "SDS-Page: Recombinant Human Indoleamine 2,3-dioxygenase/IDO Protein [NBP2-51723] - 15% SDS Page (3 ug)")
![Immunohistochemistry: Indoleamine 2,3-dioxygenase/IDO Antibody (9E5) [NBP3-26210] - Image of Indoleamine 2,3-dioxygenase/IDO Antibody (9E5) diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26210_rabbit-indoleamine-2-3-dioxygenase-ido-mab-9e5-262202414471593.jpg "Immunohistochemistry: Indoleamine 2,3-dioxygenase/IDO Antibody (9E5) [NBP3-26210] - Image of Indoleamine 2,3-dioxygenase/IDO Antibody (9E5) diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
