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![Immunocytochemistry/Immunofluorescence: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - IGF2 antibody [HL1982] detects IGF2 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGF2 stained by IGF2 antibody [HL1982] (NBP3-25520) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25520_rabbit-igf-ii-igf2-mab-hl1982-azide-and-bsa-free-2120249355530.jpg "Immunocytochemistry/Immunofluorescence: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - IGF2 antibody [HL1982] detects IGF2 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGF2 stained by IGF2 antibody [HL1982] (NBP3-25520) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Western Blot: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (NBP3-25520) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25520_rabbit-igf-ii-igf2-mab-hl1982-azide-and-bsa-free-2120249425473.jpg)
![Western Blot: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - Recombinant IGF2 protein were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (NBP3-25520) diluted at 1:2000. The HRP-conjated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25520_rabbit-igf-ii-igf2-mab-hl1982-azide-and-bsa-free-2120249413315.jpg)
![Western Blot: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - Mouse plasma (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (NBP3-25520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25520_rabbit-igf-ii-igf2-mab-hl1982-azide-and-bsa-free-2120249371569.jpg)
![ELISA: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, mature recombinant human IGF2 protein (533.33-8.33 nM). Coated protein was probed with IGF2 antibody [HL1982] (NBP3-25520) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25520_rabbit-igf-ii-igf2-mab-hl1982-azide-and-bsa-free-212024941489.jpg)
![Western Blot: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (NBP3-25520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25520_rabbit-igf-ii-igf2-mab-hl1982-azide-and-bsa-free-2120249355596.jpg)
![IGF2 antibody [HL2937] (5 μg/mL)Detection: HRP-conjugated IGF2 antibody [HL1982] (1 μg/mL). Please notice that the detection antibody requires HRP to function as the detection antibody when paired with capture antibody.Please contact us for the conjugation service.](http://images.novusbio.com/fullsize/nbp3-25520_rabbit-igf-ii-igf2-mab-hl1982-azide-and-bsa-free-elisa-161020258491.png)
![Immunohistochemistry-Paraffin: IGF-II/IGF2 Antibody [NBP2-48510] - Staining in human placenta and tonsil tissues using NBP2-48510 antibody. Corresponding IGF2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/IGF-II-IGF2-Antibody-Immunohistochemistry-Paraffin-NBP2-48510-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: IGF-II/IGF2 Antibody [NBP2-48510] - Staining in human placenta and tonsil tissues using NBP2-48510 antibody. Corresponding IGF2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: IGF-II/IGF2 Antibody [NBP2-48510] - Staining of human placenta shows moderate cytoplasmic positivity in extra villous trophoblastic cells.](http://images.novusbio.com/fullsize/IGF-II-IGF2-Antibody-Immunohistochemistry-Paraffin-NBP2-48510-img0006.jpg)
![Immunohistochemistry-Paraffin: IGF-II/IGF2 Antibody [NBP2-48510] - Staining of human duodenum shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/IGF-II-IGF2-Antibody-Immunohistochemistry-Paraffin-NBP2-48510-img0009.jpg)
![Immunohistochemistry-Paraffin: IGF-II/IGF2 Antibody [NBP2-48510] - Staining of human kidney shows weak cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/IGF-II-IGF2-Antibody-Immunohistochemistry-Paraffin-NBP2-48510-img0010.jpg)
![Immunohistochemistry-Paraffin: IGF-II/IGF2 Antibody [NBP2-48510] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/IGF-II-IGF2-Antibody-Immunohistochemistry-Paraffin-NBP2-48510-img0011.jpg)
![Western Blot: IGF2 Overexpression Lysate (Adult Normal) [NBL1-11869] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IGF2.](http://images.novusbio.com/fullsize/IGF2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11869-img0002.jpg "Western Blot: IGF2 Overexpression Lysate (Adult Normal) [NBL1-11869] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IGF2.")
![Western Blot: IGF2 Overexpression Lysate (Adult Normal) [NBL1-11868] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IGF2.](http://images.novusbio.com/fullsize/IGF2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11868-img0002.jpg "Western Blot: IGF2 Overexpression Lysate (Adult Normal) [NBL1-11868] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IGF2.")
![SDS-Page: Recombinant Human IGF-II Protein [NBP2-59722] - Recombinant IGF-II Protein [NBP2-59722] - 98 % by SDS-PAGE and HPLC analyses.](http://images.novusbio.com/fullsize/Recombinant-Human-IGF-II-Protein-SDS-Page-NBP2-59722-img0002.jpg "SDS-Page: Recombinant Human IGF-II Protein [NBP2-59722] - Recombinant IGF-II Protein [NBP2-59722] - 98 % by SDS-PAGE and HPLC analyses.")