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![Western Blot: IGF-I R/IGF1R [p Tyr1146] Antibody [NBP3-13236] - Untreated (-) and treated (+) MCF-7 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IGF1R (phospho Tyr1131) / Insulin Receptor (phospho Tyr1146) antibody (NBP3-13236) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IGF-I-R-IGF1R-[p-Tyr1146]-Antibody-Western-Blot-NBP3-13236-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: IGF-I R/IGF1R [p Tyr1146] Antibody [NBP3-13236] - Untreated (-) and treated (+) MCF-7 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IGF1R (phospho Tyr1131) / Insulin Receptor (phospho Tyr1146) antibody (NBP3-13236) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: IGF-I R/IGF1R [p Tyr950] Antibody [NBP3-13235] - Untreated (-) and treated (+) MCF-7 whole cell extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IGF-I R/IGF1R (phospho Tyr980) antibody (NBP3-13235) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IGF-I-R-IGF1R-[p-Tyr950]-Antibody-Western-Blot-NBP3-13235-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: IGF-I R/IGF1R [p Tyr950] Antibody [NBP3-13235] - Untreated (-) and treated (+) MCF-7 whole cell extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IGF-I R/IGF1R (phospho Tyr980) antibody (NBP3-13235) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: IGF1 Receptor Overexpression Lysate (Adult Normal) [NBL1-11867] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IGF1 Receptor.](http://images.novusbio.com/fullsize/IGF1-Receptor-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11867-img0002.jpg "Western Blot: IGF1 Receptor Overexpression Lysate (Adult Normal) [NBL1-11867] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IGF1 Receptor.")