MAB4234) with an ED50 of 0.0750‑1.20 μg/mL." alt="Biotinylated Recombinant Human PSMA/FOLH1/ NAALADase I Avi-tag Fc Chimera Protein (Catalog # AVI11535) binds Human PSMA/FOLH1/ NAALADase I Antibody (MAB4234) with an ED50 of 0.0750‑1.20 μg/mL." class="big_thumb" />
MAB4234) with an ED50 of 50.0-600 ng/mL." alt="Biotinylated Recombinant Human PSMA/FOLH1/ NAALADase I Protein (Catalog # BT4234) binds Human PSMA/FOLH1/NAALADase I Antibody (MAB4234) with an ED50 of 50.0-600 ng/mL." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43872 | |||
MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). Staining was performed using our Staining Membrane-associated Proteins protocol. " alt="LNCaP human prostate cancer cell line was stained with Mouse Anti-Human PSMA/FOLH1 Monoclonal Antibody (Catalog # MAB42342, filled histogram) or Mouse IgG2A isotype control antibody (MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB42342G-100UG | |||
| Carrier Free | FAB42342S-100UG | |||
| Carrier Free | FAB42342V-100UG | |||
| Carrier Free | FAB42342N-100UG | |||
| Carrier Free | FAB42342T-100UG | |||
| Carrier Free | FAB42342R-100UG | |||
| Carrier Free | FAB42342U-100UG | |||
| Carrier Free | MAB42342-SP | |||
![Immunohistochemistry-Paraffin: PSMA/FOLH1/NAALADase I Antibody (CL9089) [NBP2-88924] - Staining of human prostate cancer shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PSMA-FOLH1-NAALADase-I-Antibody-CL9089-Immunohistochemistry-Paraffin-NBP2-88924-img0004.jpg "Immunohistochemistry-Paraffin: PSMA/FOLH1/NAALADase I Antibody (CL9089) [NBP2-88924] - Staining of human prostate cancer shows strong cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: PSMA/FOLH1/NAALADase I Antibody (CL9089) [NBP2-88924] - Staining of human duodenum shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/PSMA-FOLH1-NAALADase-I-Antibody-CL9089-Immunohistochemistry-Paraffin-NBP2-88924-img0001.jpg)
![Immunohistochemistry-Paraffin: PSMA/FOLH1/NAALADase I Antibody (CL9089) [NBP2-88924] - Staining of human liver shows weak membranous positivity in hepatocytes.](http://images.novusbio.com/fullsize/PSMA-FOLH1-NAALADase-I-Antibody-CL9089-Immunohistochemistry-Paraffin-NBP2-88924-img0002.jpg)
![Immunohistochemistry-Paraffin: PSMA/FOLH1/NAALADase I Antibody (CL9089) [NBP2-88924] - Staining of human placenta shows no positivity in trophoblastic cells as expected.](http://images.novusbio.com/fullsize/PSMA-FOLH1-NAALADase-I-Antibody-CL9089-Immunohistochemistry-Paraffin-NBP2-88924-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PSMA/FOLH1/NAALADase I Antibody (CL9089) [NBP2-88924] - Analysis in human duodenum and placenta tissues using NBP2-88924 antibody. Corresponding FOLH1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PSMA-FOLH1-NAALADase-I-Antibody-CL9089-Immunohistochemistry-Paraffin-NBP2-88924-img0005.jpg)
![Immunohistochemistry: PSMA/FOLH1/NAALADase I Antibody (PD00-72) [NBP3-32862] - Immunofluorescence analysis of paraffin-embedded human prostate cancer tissue labeling PSMA/FOLH1/NAALADase I with Rabbit anti-PSMA/FOLH1/NAALADase I antibody (NBP3-32862) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32862, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32862_rabbit-psma-folh1-naaladase-i-mab-pd00-72-298202414262665.jpg "Immunohistochemistry: PSMA/FOLH1/NAALADase I Antibody (PD00-72) [NBP3-32862] - Immunofluorescence analysis of paraffin-embedded human prostate cancer tissue labeling PSMA/FOLH1/NAALADase I with Rabbit anti-PSMA/FOLH1/NAALADase I antibody (NBP3-32862) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32862, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: PSMA/FOLH1/NAALADase I Antibody (PD00-72) [NBP3-32862] - Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-PSMA/FOLH1/NAALADase I antibody (NBP3-32862) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32862) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32862_rabbit-psma-folh1-naaladase-i-mab-pd00-72-298202414115446.jpg)
![Immunocytochemistry/ Immunofluorescence: PSMA/FOLH1/NAALADase I Antibody (PD00-72) [NBP3-32862] - Immunocytochemistry analysis of LNCaP (positive) and PC-3M (negative) cells labeling PSMA/FOLH1/NAALADase I with Rabbit anti-PSMA/FOLH1/NAALADase I/FOLH1/NAALADase I antibody (NBP3-32862) at 1/100 dilution.<br/><br/> Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PSMA/FOLH1/NAALADase I antibody (NBP3-32862) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32862_rabbit-psma-folh1-naaladase-i-mab-pd00-72-2920241048486.jpg)
![Western blot analysis on different lysates with NBP3-32862 at 1/1,000 dilution. Lane 1: LNCaP cell lysate Lane 2: 22RV1 cell lysate Lane 3: PC-3M cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 84 kDa Observed band size: 100/200 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32862 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32862_-western-blot-639204595245977826.jpg)
![SDS-Page: Recombinant Human PSMA/FOLH1/NAALADase I His Protein [NBP3-12866] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain](http://images.novusbio.com/fullsize/Recombinant-Human-PSMA-FOLH1-NAALADase-I-His-Protein-SDS-Page-NBP3-12866-img0001.jpg "SDS-Page: Recombinant Human PSMA/FOLH1/NAALADase I His Protein [NBP3-12866] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain")
![Western Blot: PSMA/FOLH1/NAALADase I Antibody (k1H7) [NBP1-04338] - The Cell lysate of LNCaP(30ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with NBP1-04338. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/PSMA-FOLH1-NAALADase-I-Antibody-k1H7-Western-Blot-NBP1-04338-img0009.jpg "Western Blot: PSMA/FOLH1/NAALADase I Antibody (k1H7) [NBP1-04338] - The Cell lysate of LNCaP(30ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with NBP1-04338. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Immunocytochemistry/Immunofluorescence: PSMA/FOLH1/NAALADase I Antibody (k1H7) [NBP1-04338] - PSMA in LNCaP cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PSMA antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/PSMA-FOLH1-NAALADase-I-Antibody-k1H7-Immunocytochemistry-Immunofluorescence-NBP1-04338-img0006.jpg)
![Immunohistochemistry: PSMA/FOLH1/NAALADase I Antibody (JE42-45) [NBP3-32861] - Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-PSMA/FOLH1/NAALADase I antibody (NBP3-32861) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32861) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32861_rabbit-psma-folh1-naaladase-i-mab-je42-45-298202414262630.jpg "Immunohistochemistry: PSMA/FOLH1/NAALADase I Antibody (JE42-45) [NBP3-32861] - Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-PSMA/FOLH1/NAALADase I antibody (NBP3-32861) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32861) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western blot analysis on different lysates with NBP3-32861 at 1/1,000 dilution. Lane 1: LNCaP cell lysate Lane 2: 22RV1 cell lysate Lane 3: PC-3M cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 84 kDa Observed band size: 100/200 kDa Exposure time: 1 minute 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32861 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32861_-western-blot-639204595239106379.jpg)
