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![Western Blot: AKT1 [p Thr450] Antibody (S07-4A7) [NBP3-19824] - Western blot detection of phospho-AKT1 (Thr450) in U2OS, C6 cell lysates using NBP3-19824 (1:1000 diluted). Predicted band size: 56kDa. Observed band size: 56kDa.](http://images.novusbio.com/fullsize/AKT1-p-Thr450-Antibody-S07-4A7-Western-Blot-NBP3-19824-img0001.jpg "Western Blot: AKT1 [p Thr450] Antibody (S07-4A7) [NBP3-19824] - Western blot detection of phospho-AKT1 (Thr450) in U2OS, C6 cell lysates using NBP3-19824 (1:1000 diluted). Predicted band size: 56kDa. Observed band size: 56kDa.")
![Western Blot: AKT1 [p Ser 129] Antibody (SR2047) [NBP3-21567] - Western blot analysis using NBP3-21567 in (1) MCF-7 cell lysate treated with Alkaline Phosphatase; (2) MCF-7 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21567_rabbit-akt1-p-ser-129-mab-sr2047-5720231754841.jpg "Western Blot: AKT1 [p Ser 129] Antibody (SR2047) [NBP3-21567] - Western blot analysis using NBP3-21567 in (1) MCF-7 cell lysate treated with Alkaline Phosphatase; (2) MCF-7 cell lysate.")
![Western Blot: AKT1 [p Ser 124] Antibody (SR2049) [NBP3-21568] - Western blot analysis using NBP3-21568 in (1) MCF-7 cell treated with Alkaline Phosphatase lysate; (2) MCF-7 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21568_rabbit-akt1-p-ser-124-mab-sr2049-67202310623.jpg "Western Blot: AKT1 [p Ser 124] Antibody (SR2049) [NBP3-21568] - Western blot analysis using NBP3-21568 in (1) MCF-7 cell treated with Alkaline Phosphatase lysate; (2) MCF-7 cell lysate.")
![Western Blot: AKT1 Antibody (CL0899) [NBP2-52957] - WB using AKT1 antibody. Lane 1: Marker [kDa], Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/AKT1-Antibody-CL0899-Western-Blot-NBP2-52957-img0001.jpg "Western Blot: AKT1 Antibody (CL0899) [NBP2-52957] - WB using AKT1 antibody. Lane 1: Marker [kDa], Lane 2: Human cell line RT-4")
![Western Blot: AKT [p Thr308] Antibody (2O2W6) [NBP3-16956] - Western blot analysis of extracts of various cell lines, using Phospho-Akt-T308 antibody (NBP3-16956) at 1:1000 dilution.NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/AKT-p-Thr308-Antibody-2O2W6-Western-Blot-NBP3-16956-img0001.jpg "Western Blot: AKT [p Thr308] Antibody (2O2W6) [NBP3-16956] - Western blot analysis of extracts of various cell lines, using Phospho-Akt-T308 antibody (NBP3-16956) at 1:1000 dilution.NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: AKT1 Overexpression Lysate (Adult Normal) [NBP2-04280] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AKT1.](http://images.novusbio.com/fullsize/AKT1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04280-img0001.jpg "Western Blot: AKT1 Overexpression Lysate (Adult Normal) [NBP2-04280] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AKT1.")
![Western Blot: AKT1 Overexpression Lysate [NBP2-09148] - Left lane: Empty vector transfected control cell lysate (HEK293 cell lysate); Right lane: Over-expression Lysate for AKT1.](http://images.novusbio.com/fullsize/AKT1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09148-img0001.jpg "Western Blot: AKT1 Overexpression Lysate [NBP2-09148] - Left lane: Empty vector transfected control cell lysate (HEK293 cell lysate); Right lane: Over-expression Lysate for AKT1.")
![Western Blot: AKT1 [p Ser129] Antibody (7M4I8) [NBP3-16908] - Western blot analysis of extracts of MCF7 cells, using AKT1 Rabbit mAb (NBP3-16908) at 1:1000 dilution.MCF7 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/AKT1-p-Ser129-Antibody-7M4I8-Western-Blot-NBP3-16908-img0001.jpg "Western Blot: AKT1 [p Ser129] Antibody (7M4I8) [NBP3-16908] - Western blot analysis of extracts of MCF7 cells, using AKT1 Rabbit mAb (NBP3-16908) at 1:1000 dilution.MCF7 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: AKT1 Overexpression Lysate [NBL1-07441] - Left lane: Empty vector transfected control cell lysate (HEK293 cell lysate). Right lane: Over-expression lysate for AKT1.](http://images.novusbio.com/fullsize/AKT1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07441-img0002.jpg "Western Blot: AKT1 Overexpression Lysate [NBL1-07441] - Left lane: Empty vector transfected control cell lysate (HEK293 cell lysate). Right lane: Over-expression lysate for AKT1.")
![Western Blot: AKT1 Overexpression Lysate [NBP2-08736] - Left lane: Empty vector transfected control cell lysate (HEK293 cell lysate); Right lane: Over-expression Lysate for AKT1.](http://images.novusbio.com/fullsize/AKT1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08736-img0001.jpg "Western Blot: AKT1 Overexpression Lysate [NBP2-08736] - Left lane: Empty vector transfected control cell lysate (HEK293 cell lysate); Right lane: Over-expression Lysate for AKT1.")
![Western Blot: AKT [p Thr342] Antibody [NBP2-60772] - Western blot of Drosophila lysate showing specific labelingof the ~68 kDa AKT protein phosphorylated at Thr342 in thefirst lane (-). Phosphospecificity is shown in the secondlane (+) where the immunolabeling is completely eliminatedby blot treatment with lambda phosphatase (Lambda-Ptase, 1200units for 30 minutes).](http://images.novusbio.com/fullsize/AKT-[p-Thr342]-Antibody-Western-Blot-NBP2-60772-img0001.jpg "Western Blot: AKT [p Thr342] Antibody [NBP2-60772] - Western blot of Drosophila lysate showing specific labelingof the ~68 kDa AKT protein phosphorylated at Thr342 in thefirst lane (-). Phosphospecificity is shown in the secondlane (+) where the immunolabeling is completely eliminatedby blot treatment with lambda phosphatase (Lambda-Ptase, 1200units for 30 minutes).")