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![Western Blot: AKT [p Thr308] Antibody (2O2W6) [NBP3-16956] - Western blot analysis of extracts of various cell lines, using Phospho-Akt-T308 antibody (NBP3-16956) at 1:1000 dilution.NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/AKT-p-Thr308-Antibody-2O2W6-Western-Blot-NBP3-16956-img0001.jpg "Western Blot: AKT [p Thr308] Antibody (2O2W6) [NBP3-16956] - Western blot analysis of extracts of various cell lines, using Phospho-Akt-T308 antibody (NBP3-16956) at 1:1000 dilution.NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: AKT [p Thr342] Antibody [NBP2-60772] - Western blot of Drosophila lysate showing specific labelingof the ~68 kDa AKT protein phosphorylated at Thr342 in thefirst lane (-). Phosphospecificity is shown in the secondlane (+) where the immunolabeling is completely eliminatedby blot treatment with lambda phosphatase (Lambda-Ptase, 1200units for 30 minutes).](http://images.novusbio.com/fullsize/AKT-[p-Thr342]-Antibody-Western-Blot-NBP2-60772-img0001.jpg "Western Blot: AKT [p Thr342] Antibody [NBP2-60772] - Western blot of Drosophila lysate showing specific labelingof the ~68 kDa AKT protein phosphorylated at Thr342 in thefirst lane (-). Phosphospecificity is shown in the secondlane (+) where the immunolabeling is completely eliminatedby blot treatment with lambda phosphatase (Lambda-Ptase, 1200units for 30 minutes).")
![Western Blot: AKT1 Overexpression Lysate [NBL1-07441] - Left lane: Empty vector transfected control cell lysate (HEK293 cell lysate). Right lane: Over-expression lysate for AKT1.](http://images.novusbio.com/fullsize/AKT1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07441-img0002.jpg "Western Blot: AKT1 Overexpression Lysate [NBL1-07441] - Left lane: Empty vector transfected control cell lysate (HEK293 cell lysate). Right lane: Over-expression lysate for AKT1.")
![Western Blot: AKT1 Overexpression Lysate [NBP2-08736] - Left lane: Empty vector transfected control cell lysate (HEK293 cell lysate); Right lane: Over-expression Lysate for AKT1.](http://images.novusbio.com/fullsize/AKT1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08736-img0001.jpg "Western Blot: AKT1 Overexpression Lysate [NBP2-08736] - Left lane: Empty vector transfected control cell lysate (HEK293 cell lysate); Right lane: Over-expression Lysate for AKT1.")
![Western Blot: AKT1 Overexpression Lysate (Adult Normal) [NBP2-04280] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AKT1.](http://images.novusbio.com/fullsize/AKT1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04280-img0001.jpg "Western Blot: AKT1 Overexpression Lysate (Adult Normal) [NBP2-04280] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AKT1.")
![Western Blot: AKT1 Overexpression Lysate [NBP2-09148] - Left lane: Empty vector transfected control cell lysate (HEK293 cell lysate); Right lane: Over-expression Lysate for AKT1.](http://images.novusbio.com/fullsize/AKT1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09148-img0001.jpg "Western Blot: AKT1 Overexpression Lysate [NBP2-09148] - Left lane: Empty vector transfected control cell lysate (HEK293 cell lysate); Right lane: Over-expression Lysate for AKT1.")
![Western Blot: AKT [p Ser308] Antibody (0002) [NBP3-23519] - Western blot analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with PDGFA (5 ug/mL, 5 min) (line B) using NBP3-23519 at 1:5000 dilution.](http://images.novusbio.com/fullsize/nbp3-23519_rabbit-akt-mab-0002-azide-and-bsa-free-235202514331565.png "Western Blot: AKT [p Ser308] Antibody (0002) [NBP3-23519] - Western blot analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with PDGFA (5 ug/mL, 5 min) (line B) using NBP3-23519 at 1:5000 dilution.")
![Western Blot: AKT1 [p Ser129] Antibody (7M4I8) [NBP3-16908] - Western blot analysis of extracts of MCF7 cells, using AKT1 Rabbit mAb (NBP3-16908) at 1:1000 dilution.MCF7 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/AKT1-p-Ser129-Antibody-7M4I8-Western-Blot-NBP3-16908-img0001.jpg "Western Blot: AKT1 [p Ser129] Antibody (7M4I8) [NBP3-16908] - Western blot analysis of extracts of MCF7 cells, using AKT1 Rabbit mAb (NBP3-16908) at 1:1000 dilution.MCF7 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3min.")