| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-206-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52671-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47743-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27815-50ul | |||
![Western Blot: EGFR [p Tyr1045] Antibody (PSH03-28) [NBP3-32760] - Western blot analysis of EGFR on different lysates with Rabbit anti-EGFR antibody (NBP3-32760) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: A431 treated with 100ng/mL EGF for 30 minutes cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 134 kDa Observed band size: 170 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32760) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32760_rabbit-egfr-p-tyr1045-mab-psh03-28-288202412461681.jpg "Western Blot: EGFR [p Tyr1045] Antibody (PSH03-28) [NBP3-32760] - Western blot analysis of EGFR on different lysates with Rabbit anti-EGFR antibody (NBP3-32760) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: A431 treated with 100ng/mL EGF for 30 minutes cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 134 kDa Observed band size: 170 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32760) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: EGFR [p Tyr1045] Antibody (PSH03-28) [NBP3-32760] - Immunocytochemistry analysis of A431 treated with or without 100ng/mL EGF for 60 minutes cells labeling EGFR with Rabbit anti-EGFR antibody (NBP3-32760) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EGFR antibody (NBP3-32760) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32760_rabbit-egfr-p-tyr1045-mab-psh03-28-29202410484836.jpg)
![Flow Cytometry: EGFR [p Tyr1045] Antibody (PSH03-28) [NBP3-32760] - Flow cytometric analysis of A431 treated with or without 100ng/mL EGF for 60 minutes cells labeling EGFR. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32760, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32760_rabbit-egfr-p-tyr1045-mab-psh03-28-29202416453235.jpg)
![Western Blot: EGFR [p Tyr1148] Antibody [NBP3-13263] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1148) antibody (NBP3-13263) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1148]-Antibody-Western-Blot-NBP3-13263-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Tyr1148] Antibody [NBP3-13263] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1148) antibody (NBP3-13263) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: EGFR [p Tyr1148] Antibody [NBP3-13263] - EGFR (phospho Tyr1148) antibody detects EGFR (phospho Tyr1148) protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR (phospho Tyr1148) protein stained by EGFR (phospho Tyr1148) antibody (NBP3-13263) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1148]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13263-img0002.jpg)
![Western Blot: EGFR [p Tyr992] Antibody [NBP3-13228] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr992) antibody (NBP3-13228) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr992]-Antibody-Western-Blot-NBP3-13228-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Tyr992] Antibody [NBP3-13228] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr992) antibody (NBP3-13228) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: EGFR [p Tyr992] Antibody [NBP3-13228] - EGFR (phospho Tyr992) antibody detects EGFR (phospho Tyr992) protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR (phospho Tyr992) protein stained by EGFR (phospho Tyr992) antibody (NBP3-13228) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr992]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13228-img0002.jpg)
![Western Blot: EGFR [p Tyr992] Antibody [NBP3-13265] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr992) antibody (NBP3-13265) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr992]-Antibody-Western-Blot-NBP3-13265-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Tyr992] Antibody [NBP3-13265] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr992) antibody (NBP3-13265) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: EGFR [p Tyr992] Antibody [NBP3-13265] - EGFR (phospho Tyr992) antibody detects EGFR (phospho Tyr992) protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR (phospho Tyr992) protein stained by EGFR (phospho Tyr992) antibody (NBP3-13265) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr992]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13265-img0002.jpg)
![Western Blot: EGFR Antibody (HL1022) - L858R Mutant [NBP3-13683] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [HL1022] (NBP3-13683) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13683_rabbit-egfr-mab-hl1022-l858r-mutant-910202420115098.jpg "Western Blot: EGFR Antibody (HL1022) - L858R Mutant [NBP3-13683] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [HL1022] (NBP3-13683) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: EGFR Antibody (HL1022) - L858R Mutant [NBP3-13683] - Various whole cell extracts (30 | 15 | 15 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [HL1022] (NBP3-13683) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13683_rabbit-egfr-mab-hl1022-l858r-mutant-91020242063323.jpg)
![Immunohistochemistry-Paraffin: EGFR Antibody (31G7 + GFR1195) - IHC-Prediluted [NBP2-48218] - Formalin-fixed, paraffin-embedded human Placenta stained with EGFR Monoclonal Antibody (31G7 + GFR1195).](http://images.novusbio.com/fullsize/EGFR-Antibody-31G7-+-GFR1195-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48218-img0001.jpg "Immunohistochemistry-Paraffin: EGFR Antibody (31G7 + GFR1195) - IHC-Prediluted [NBP2-48218] - Formalin-fixed, paraffin-embedded human Placenta stained with EGFR Monoclonal Antibody (31G7 + GFR1195).")
![Western Blot: EGFR [p Ser 695] Antibody (SR1251) [NBP3-27815] - Western blot analysis of A431 cell lysate treated with EGF.](http://images.novusbio.com/fullsize/nbp3-27815_rabbit-egfr-p-ser-695-mab-sr1251-7520241015114.jpg "Western Blot: EGFR [p Ser 695] Antibody (SR1251) [NBP3-27815] - Western blot analysis of A431 cell lysate treated with EGF.")