| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26606-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89582-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89581-500ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF236 | |||
| Carrier Free | AF236-SP | |||
![Immunohistochemistry: EGF Antibody (18B12) [NBP3-26606] - Image of EGF Antibody (18B12) diluted at 1:100 and staining in paraffin-embedded human skin tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.66% DAB.](http://images.novusbio.com/fullsize/nbp3-26606_rabbit-egf-mab-18b12-262202414225218.jpg "Immunohistochemistry: EGF Antibody (18B12) [NBP3-26606] - Image of EGF Antibody (18B12) diluted at 1:100 and staining in paraffin-embedded human skin tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.66% DAB.")
![Flow Cytometry: EGF Antibody (18B12) [NBP3-26606] - Overlay Peak curve showing SH-SY5Y cells stained with EGF Antibody (18B12) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26606_rabbit-egf-mab-18b12-282202414183693.jpg)
![Western Blot: EGF Overexpression Lysate (Adult Normal) [NBL1-10149] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EGF.](http://images.novusbio.com/fullsize/EGF-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10149-img0002.jpg "Western Blot: EGF Overexpression Lysate (Adult Normal) [NBL1-10149] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EGF.")
![Neutralization: EGF Antibody (R001) [NBP2-89581] - Recombinant Human EGF stimulates proliferation in the Balb/c 3T3 mouse embryonic fibroblast cell line. Proliferation elicited by Recombinant Human EGF (2 ng/mL) is neutralized by increasing concentrations of Human EGF Monoclonal Antibody. The IC50 is typically 2.5-7.5 ug/mL.](http://images.novusbio.com/fullsize/EGF-Antibody-R001-Neutralization-NBP2-89581-img0001.jpg "Neutralization: EGF Antibody (R001) [NBP2-89581] - Recombinant Human EGF stimulates proliferation in the Balb/c 3T3 mouse embryonic fibroblast cell line. Proliferation elicited by Recombinant Human EGF (2 ng/mL) is neutralized by increasing concentrations of Human EGF Monoclonal Antibody. The IC50 is typically 2.5-7.5 ug/mL.")