NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="Cathepsin D was detected in immersion fixed U937 human histiocytic lymphoma cell line using Mouse Anti-Human Cathepsin D Monoclonal Antibody (Catalog # MAB10141) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB10141-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB10142-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB10143-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90432-50ul | |||
![ELISA: Mouse Cathepsin D ELISA Kit (Colorimetric) [NBP2-67258] - Samples were spiked with high concentrations of Mouse Cathepsin D and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-67258_mouse-cathepsin-d-elisa-kit-colorimetric-132202416243475.jpg "ELISA: Mouse Cathepsin D ELISA Kit (Colorimetric) [NBP2-67258] - Samples were spiked with high concentrations of Mouse Cathepsin D and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Cathepsin D ELISA Kit (Colorimetric) [NBP2-67258] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-67258_mouse-cathepsin-d-elisa-kit-colorimetric-4320249124858.jpg)
![Western Blot: Cathepsin D Antibody (102) [NBP2-90432] - Anti-Cathepsin D rabbit monoclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 45 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Cathepsin-D-Antibody-102-Western-Blot-NBP2-90432-img0002.jpg "Western Blot: Cathepsin D Antibody (102) [NBP2-90432] - Anti-Cathepsin D rabbit monoclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 45 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: Cathepsin D Antibody (102) [NBP2-90432] - Mouse Cathepsin D was immunoprecipitated using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. 0.5 ul anti-Mouse Cathepsin D rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse Cathepsin D rabbit monoclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 45 kDa](http://images.novusbio.com/fullsize/Cathepsin-D-Antibody-102-Immunoprecipitation-NBP2-90432-img0001.jpg)
![ELISA: Human Cathepsin D ELISA Kit (Colorimetric) [NBP2-67257] - Samples were spiked with high concentrations of Human Cathepsin D and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-67257_human-cathepsin-d-elisa-kit-colorimetric-13220241685345.jpg "ELISA: Human Cathepsin D ELISA Kit (Colorimetric) [NBP2-67257] - Samples were spiked with high concentrations of Human Cathepsin D and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Cathepsin D ELISA Kit (Colorimetric) [NBP2-67257] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-67257_human-cathepsin-d-elisa-kit-colorimetric-4320249151676.jpg)
![ELISA: Human Cathepsin D ELISA Kit (Chemiluminescence) [NBP2-67256] - Samples were spiked with high concentrations of Human Cathepsin D and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-67256_human-cathepsin-d-elisa-kit-chemiluminescence-13220241613057.jpg "ELISA: Human Cathepsin D ELISA Kit (Chemiluminescence) [NBP2-67256] - Samples were spiked with high concentrations of Human Cathepsin D and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Cathepsin D ELISA Kit (Chemiluminescence) [NBP2-67256] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-67256_human-cathepsin-d-elisa-kit-chemiluminescence-4320249163336.jpg)
![Western Blot: Cathepsin D Overexpression Lysate (Adult Normal) [NBL1-09590] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cathepsin D.](http://images.novusbio.com/fullsize/Cathepsin-D-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09590-img0002.jpg "Western Blot: Cathepsin D Overexpression Lysate (Adult Normal) [NBL1-09590] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cathepsin D.")
