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![Western Blot: Histone H3 [ac Lys36] Antibody [NBP2-59273] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K36ac. The antibody was diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys36]-Antibody-Western-Blot-NBP2-59273-img0001.jpg "Western Blot: Histone H3 [ac Lys36] Antibody [NBP2-59273] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K36ac. The antibody was diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys36] Antibody [NBP2-59273] - ChIP assays were performed using human HeLa cells, the antibody against H3K36ac and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for a region approximately 1 kb upstream of the ACTB promoter and for the GAPDH promoter, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys36]-Antibody-Chromatin-Immunoprecipitation-NBP2-59273-img0004.jpg)
![Dot Blot: Histone H3 [ac Lys36] Antibody [NBP2-59273] - To test the cross reactivity of the antibody against H3K36ac, a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K36. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:10,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys36]-Antibody-Dot-Blot-NBP2-59273-img0002.jpg)
![ELISA: Histone H3 [ac Lys36] Antibody [NBP2-59273] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K36ac in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:10,000.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys36]-Antibody-ELISA-NBP2-59273-img0003.jpg)
![Western Blot: Histone H3 [Trimethyl Lys64] Antibody [NBP2-59227] - Western blot was performed on histone extracts (30 ug) from HeLa cells using the antibody against H3K64me3 . The antibody was diluted 1:100 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys64]-Antibody-Western-Blot-NBP2-59227-img0001.jpg "Western Blot: Histone H3 [Trimethyl Lys64] Antibody [NBP2-59227] - Western blot was performed on histone extracts (30 ug) from HeLa cells using the antibody against H3K64me3 . The antibody was diluted 1:100 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys64] Antibody [NBP2-59227] - ChIP assays were performed using human K562 cells, the antibody against H3K64me3 and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP-seq kit on sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active GAPDH and EIF4A2 genes, used as negative controls, and for the Sat2 and Sata satellite repeats, used as positive controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys64]-Antibody-Chromatin-Immunoprecipitation-NBP2-59227-img0003.jpg)
![ELISA: Histone H3 [Trimethyl Lys64] Antibody [NBP2-59227] - To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against H3K64me3 in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:5,500.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys64]-Antibody-ELISA-NBP2-59227-img0002.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys64] Antibody [NBP2-59227] - To check the specificity of the antibody against H3K64me3 a Dot Blot was performed with peptides containing different modifications of histone H3 and H4 or the unmodified H3K64 sequence. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:5,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys64]-Antibody-Dot-Blot-NBP2-59227-img0005.jpg)
![Western Blot: Histone H3 [Monomethyl Lys9] Antibody [NBP2-59204] - Histone H3 Antibody [NBP2-59204] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K9me1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NBP2-59204-img0001.jpg "Western Blot: Histone H3 [Monomethyl Lys9] Antibody [NBP2-59204] - Histone H3 Antibody [NBP2-59204] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K9me1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [Monomethyl Lys9] Antibody [NBP2-59204] - Histone H3 Antibody [NBP2-59204] - ChIP assays were performed using human HeLa cells, the antibody directed against H3K9me1 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 10,000 cells. Respectively 1 and 5 ug of the antibody and 5 ug of IgG (negative IP control) were used per ChIP experiment. QPCR was performed with primers for the GAPDH promoter and for the inactive gene MYOD. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K9me1 is preferably present at silent genes.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Chromatin-Immunoprecipitation-NBP2-59204-img0004.jpg)
![Dot Blot: Histone H3 [Monomethyl Lys9] Antibody [NBP2-59204] - Histone H3 Antibody [NBP2-59204] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K9me1 with peptides containing other modifications and the unmodified sequence of histone H3. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Dot-Blot-NBP2-59204-img0002.jpg)
![ELISA: Histone H3 [Monomethyl Lys9] Antibody [NBP2-59204] - Histone H3 Antibody [NBP2-59204] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human H3K9me1, crude serum and flow through in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:68,000.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-ELISA-NBP2-59204-img0003.jpg)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys56] Antibody [NBP2-59214] - ChIP assays were performed using human HeLa cells, the antibody against H3K56ac and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and, 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for a region approximately 1 kb upstream of the GAPDH promoter and for the EIF4A2 promoter, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys56]-Antibody-Chromatin-Immunoprecipitation-NBP2-59214-img0002.jpg "Chromatin Immunoprecipitation: Histone H3 [ac Lys56] Antibody [NBP2-59214] - ChIP assays were performed using human HeLa cells, the antibody against H3K56ac and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and, 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for a region approximately 1 kb upstream of the GAPDH promoter and for the EIF4A2 promoter, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).")
![ELISA: Histone H3 [ac Lys56] Antibody [NBP2-59214] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K56ac in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:15,300.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys56]-Antibody-ELISA-NBP2-59214-img0001.jpg)
![Dot Blot: Histone H3 [ac Lys56] Antibody [NBP2-59214] - To test the cross reactivity of the antibody against H3K56ac, a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K56. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys56]-Antibody-Dot-Blot-NBP2-59214-img0004.jpg)
![Western Blot: Histone H3 [Methyl Lys4] Antibody (C.15200150) [NBP2-59284] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K4me1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Methyl-Lys4-Antibody-C-15200150-Western-Blot-NBP2-59284-img0001.jpg "Western Blot: Histone H3 [Methyl Lys4] Antibody (C.15200150) [NBP2-59284] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K4me1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Methyl Lys4] Antibody (C.15200150) [NBP2-59284] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-Methyl-Lys4-Antibody-C-15200150-Immunofluorescence-NBP2-59284-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Methyl Lys4] Antibody (C.15200150) [NBP2-59284] - ChIP assays were performed using HeLa cells, the monoclonal antibody against H3K4me1 and optimized PCR primer sets for qPCR. Chromatin was sheared with the antibody consisting of 1, 5 and 10 ug per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoter and the coding region of the GAPDH gene, and for the RPL10 and HBB promoters. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-Methyl-Lys4-Antibody-C-15200150-Chromatin-Immunoprecipitation-NBP2-59284-img0003.jpg)
![ELISA: Histone H3 [Methyl Lys4] Antibody (C.15200150) [NBP2-59284] - Cross reactivity of the antibody directed against H3K4me1. To test the specificity an ELISA was performed using a serial dilution of the antibody against H3K4me1. The wells were coated with peptides containing the unmodified H3K4 as well as the mono-, di- and trimethylated H3K4 and the monomethylated H3K9. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-Methyl-Lys4-Antibody-C-15200150-ELISA-NBP2-59284-img0002.jpg)
![Western Blot: Histone H3 [Trimethyl Lys27] Antibody (BT164) - Chimeric [NBP3-12020] - Nuclear lysate of HeLa(A) (0.0003ug/ml), Jurkat(B) (0.0003ug/ml) and K562(C) (0.01ug/ml) (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of BT164 (NBP3-12020) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys27-Antibody-BT164-Chimeric-Western-Blot-NBP3-12020-img0001.jpg "Western Blot: Histone H3 [Trimethyl Lys27] Antibody (BT164) - Chimeric [NBP3-12020] - Nuclear lysate of HeLa(A) (0.0003ug/ml), Jurkat(B) (0.0003ug/ml) and K562(C) (0.01ug/ml) (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of BT164 (NBP3-12020) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys27] Antibody (BT164) - Chimeric [NBP3-12020] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of BT164 (NBP3-12020) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right, top-bottom NBP3-12020, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys27-Antibody-BT164-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12020-img0002.jpg)
![Flow Cytometry: Histone H3 [Trimethyl Lys27] Antibody (BT164) - Chimeric - Azide and BSA Free [NBP3-12020] - HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12020 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12020_rabbit-monoclonal-histone-h3-trimethyl-lys27-antibody-bt164-chimeric-7420252211303.png)
![Western Blot: Histone H3 [Monomethyl Lys14] Antibody [NBP3-15944] - Western blot analysis of extracts of various cell lines, using Histone H3 antibody (NBP3-15944) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys14]-Antibody-Western-Blot-NBP3-15944-img0003.jpg "Western Blot: Histone H3 [Monomethyl Lys14] Antibody [NBP3-15944] - Western blot analysis of extracts of various cell lines, using Histone H3 antibody (NBP3-15944) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Monomethyl Lys14] Antibody [NBP3-15944] - Immunofluorescence analysis of U-2 OS cells using Histone H3 pAb (NBP3-15944) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys14]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15944-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Monomethyl Lys14] Antibody [NBP3-15944] - Immunofluorescence analysis of NIH-3T3 cells using Histone H3 pAb (NBP3-15944) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys14]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15944-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H3 [p Thr32] Antibody [NBP3-16009] - Rat ovary using Histone H3 antibody (NBP3-16009) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr32]-Antibody-Immunohistochemistry-Paraffin-NBP3-16009-img0002.jpg "Immunohistochemistry-Paraffin: Histone H3 [p Thr32] Antibody [NBP3-16009] - Rat ovary using Histone H3 antibody (NBP3-16009) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.")
![Immunohistochemistry-Paraffin: Histone H3 [p Thr32] Antibody [NBP3-16009] - Human tonsil using Histone H3 antibody (NBP3-16009) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr32]-Antibody-Immunohistochemistry-Paraffin-NBP3-16009-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H3 [p Thr32] Antibody [NBP3-16009] - Mouse brain using Histone H3 antibody (NBP3-16009) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr32]-Antibody-Immunohistochemistry-Paraffin-NBP3-16009-img0003.jpg)
![Other Histone H3 [Monomethyl Lys4] Antibody Blocking Peptide](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys4]-Peptide-Chromatin-Immunoprecipitation-NB21-1021PEP-img0002.jpg "Other Histone H3 [Monomethyl Lys4] Antibody Blocking Peptide")
![Other Histone H3 [Monomethyl Lys4, p Thr6] Antibody Blocking Peptide](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys4-p-Thr6]-Peptide-Chromatin-Immunoprecipitation-NB21-1037PEP-img0002.jpg "Other Histone H3 [Monomethyl Lys4, p Thr6] Antibody Blocking Peptide")
![Other Histone H3 [Dimethyl Lys36] Antibody Blocking Peptide](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys36]-Peptide-Chromatin-Immunoprecipitation-NB21-1252PEP-img0002.jpg "Other Histone H3 [Dimethyl Lys36] Antibody Blocking Peptide")
![Other Histone H3 [Trimethyl Lys18] Antibody Blocking Peptide](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys18]-Peptide-Chromatin-Immunoprecipitation-NB21-1143PEP-img0002.jpg "Other Histone H3 [Trimethyl Lys18] Antibody Blocking Peptide")