| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12019-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26036 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26012 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26024 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12018-0.025mg | |||
![Western Blot: Histone H4 [ac Lys8] Antibody (C.15200217) [NBP2-59240] - Histone extracts (15 ug) from HeLa cells were analyzed using the antibody against H4K8ac diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys8-Antibody-C-15200217-Western-Blot-NBP2-59240-img0001.jpg "Western Blot: Histone H4 [ac Lys8] Antibody (C.15200217) [NBP2-59240] - Histone extracts (15 ug) from HeLa cells were analyzed using the antibody against H4K8ac diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.")
![Chromatin Immunoprecipitation: Histone H4 [ac Lys8] Antibody (C.15200217) [NBP2-59240] - Analysis was performed using HeLa cells, the monoclonal antibody against H4K8ac and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the EIF4A2 gene, used as a positive control, and for the Sat2 satellite repeat, used as a negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys8-Antibody-C-15200217-Chromatin-Immunoprecipitation-NBP2-59240-img0002.jpg)
![Dot Blot: Histone H4 [ac Lys8] Antibody (C.15200217) [NBP2-59240] - To test the cross reactivity of the antibody against H4K8ac, analysis was performed with peptides containing other acetylations and unmodified sequences of histone H4. 100 to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys8-Antibody-C-15200217-Dot-Blot-NBP2-59240-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys12, ac Lys16, ac Lys8] Antibody (KM-2) - Chimeric [NBP3-12019] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of KM-2 (NBP3-12019) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right, top-bottom NBP3-12019, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys12-ac-Lys16-ac-Lys8-Antibody-KM-2-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12019-img0001.jpg "Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys12, ac Lys16, ac Lys8] Antibody (KM-2) - Chimeric [NBP3-12019] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of KM-2 (NBP3-12019) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right, top-bottom NBP3-12019, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: Histone H4 [ac Lys12, ac Lys16, ac Lys8] Antibody (KM-2) - Chimeric - Azide and BSA Free [NBP3-12019] - HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12019 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12019_rabbit-monoclonal-histone-h4-ac-lys12-ac-lys16-ac-lys8-antibody-km-2-chimeric-74202522113023.png)
![Western Blot: Histone H4 [Monomethyl Lys20] Antibody [NBP2-42859] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract 15 % SDS-PAGE Histone H4 (mono methyl Lys20) antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Histone-H4-[Monomethyl-Lys20]-Antibody-Western-Blot-NBP2-42859-img0003.jpg "Western Blot: Histone H4 [Monomethyl Lys20] Antibody [NBP2-42859] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract 15 % SDS-PAGE Histone H4 (mono methyl Lys20) antibody dilution: 1:1000")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Lys20] Antibody [NBP2-42859] - Analysis of HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (mono methyl Lys20) protein stained by Histone H4 (mono methyl Lys20) antibody diluted at 1:500. Red: alpha Tubulin antibody [114], a cytoskeleton marker, stained by diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Histone-H4-[Monomethyl-Lys20]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42859-img0002.jpg)
![Western Blot: Histone H4 [ac Lys16] Antibody (RM204) - Azide and BSA Free [NBP3-26012] - Western Blot of acid extracts from: (1) HeLa cells treated with sodium butyrate; (2) HeLa cells untreated, using RM204 at 0.5 ug/mL, showed a band of Histone H4 acetylated at Lysine 16 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-26012_rabbit-histone-h4-mab-rm204-azide-and-bsa-free-2462024132637.png "Western Blot: Histone H4 [ac Lys16] Antibody (RM204) - Azide and BSA Free [NBP3-26012] - Western Blot of acid extracts from: (1) HeLa cells treated with sodium butyrate; (2) HeLa cells untreated, using RM204 at 0.5 ug/mL, showed a band of Histone H4 acetylated at Lysine 16 in HeLa cells.")
![Multiplex Immunoassay: Histone H4 [ac Lys16] Antibody (RM204) - Azide and BSA Free [NBP3-26012] - RM204 specifically reacts to Histone H4 acetylated at Lysine 16 (K16ac). No cross reactivity with unmodified Lysine 16 (K16 ctrl), acetylated Lysine 5 (K5ac), Lysine 8 (K8ac), Lysine 12 (K12ac), Lysine 20 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.](http://images.novusbio.com/fullsize/nbp3-26012_rabbit-histone-h4-mab-rm204-azide-and-bsa-free-22620242311017.png)
![Western Blot: Histone H4 [Monomethyl Lys5] Antibody (RM457) [NBP3-18844] - Western Blot of acid extracts of HeLa cells (2) and recombinant histone H4 (1), using RM457 at 0.2 ug/mL, showed a band of histone H4 monomethylated at Lysine 5 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-18844_rabbit-histone-h4-mab-rm457-1772024100323.png "Western Blot: Histone H4 [Monomethyl Lys5] Antibody (RM457) [NBP3-18844] - Western Blot of acid extracts of HeLa cells (2) and recombinant histone H4 (1), using RM457 at 0.2 ug/mL, showed a band of histone H4 monomethylated at Lysine 5 in HeLa cells.")
![Multiplex Immunoassay: Histone H4 [Monomethyl Lys5] Antibody (RM457) [NBP3-18844] - RM457 specifically reacts to Histone H4 monomethylated at Lysine 5 (K5me1). No cross reactivity with non-modified Lysine 5, or other monomethylations in histone H4.](http://images.novusbio.com/fullsize/nbp3-18844_rabbit-histone-h4-mab-rm457-17720249362621.png)
![Western Blot: Histone H4 [ac Lys16] Antibody (RM204) [NBP2-61551] - Western Blot of acid extracts from: (1) HeLa cells treated with sodium butyrate; (2) HeLa cells untreated. Using NBP2-61551, showed a band of Histone H4 acetylated at Lysine 16 in HeLa cells.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-RM204-Western-Blot-NBP2-61551-img0004.jpg "Western Blot: Histone H4 [ac Lys16] Antibody (RM204) [NBP2-61551] - Western Blot of acid extracts from: (1) HeLa cells treated with sodium butyrate; (2) HeLa cells untreated. Using NBP2-61551, showed a band of Histone H4 acetylated at Lysine 16 in HeLa cells.")
![Multiplex Immunoassay: Histone H4 [ac Lys16] Antibody (RM204) [NBP2-61551] - NBP2-61551 specifically reacts to Histone H4 acetylated at Lysine 16 (K16ac). No cross reactivity with unmodified Lysine 16 (K16 ctrl), acetylated Lysine 5 (K5ac), Lysine 8 (K8ac), Lysine 12 (K12ac), Lysine 20 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-RM204-Multiplex-Immunoassay-NBP2-61551-img0005.jpg)
![Western Blot: Histone H4 [Methyl Lys20] Antibody (RM208) [NBP2-61552] - Western Blot of acid extracts of HeLa cells (1) and recombinant Histone H4 (2), using NBP2-61552, showed a band of Histone H4 trimethylated at Lysine 20 (K20me3) in HeLa cells.](http://images.novusbio.com/fullsize/Histone-H4-Methyl-Lys20-Antibody-RM208-Western-Blot-NBP2-61552-img0004.jpg "Western Blot: Histone H4 [Methyl Lys20] Antibody (RM208) [NBP2-61552] - Western Blot of acid extracts of HeLa cells (1) and recombinant Histone H4 (2), using NBP2-61552, showed a band of Histone H4 trimethylated at Lysine 20 (K20me3) in HeLa cells.")
![Multiplex Immunoassay: Histone H4 [Methyl Lys20] Antibody (RM208) [NBP2-61552] - NBP2-61552 specifically reacts to Histone H4 trimethylated at Lysine 20 (K20me3). May slightly cross react to dimethylated Lysine 20 (K20me2) when used at higher concentrations. No cross reactivity with non-modified Lysine 20, monomethylated Lysine 20 (K20me1), or other methylations in histone H4.](http://images.novusbio.com/fullsize/Histone-H4-Methyl-Lys20-Antibody-RM208-Multiplex-Immunoassay-NBP2-61552-img0005.jpg)
![Western Blot: Histone H4 [ac Lys12] Antibody (C.15200218) [NBP2-59241] - Histone extracts (15 ug) from HeLa cells were analyzed using the antibody against H4K12ac diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys12-Antibody-C-15200218-Western-Blot-NBP2-59241-img0001.jpg "Western Blot: Histone H4 [ac Lys12] Antibody (C.15200218) [NBP2-59241] - Histone extracts (15 ug) from HeLa cells were analyzed using the antibody against H4K12ac diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.")
![Chromatin Immunoprecipitation: Histone H4 [ac Lys12] Antibody (C.15200218) [NBP2-59241] - ChIP assays were performed using HeLa cells, the monoclonal antibody against H4K12ac and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for the coding region of the EIF2S3 gene, used as a positive control, and for the Sat2 satellite repeat, used as a negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys12-Antibody-C-15200218-Chromatin-Immunoprecipitation-NBP2-59241-img0002.jpg)