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![Western Blot: ERCC8 Antibody (PSH0-86) [NBP3-32315] - Western blot analysis of ERCC8 on different lysates with Rabbit anti-ERCC8 antibody (NBP3-32315) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: HepG2 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 44 kDa Observed band size: 55 kDa Exposure time: 27 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32315) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32315_rabbit-ercc8-mab-psh0-86-278202416285297.jpg "Western Blot: ERCC8 Antibody (PSH0-86) [NBP3-32315] - Western blot analysis of ERCC8 on different lysates with Rabbit anti-ERCC8 antibody (NBP3-32315) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: HepG2 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 44 kDa Observed band size: 55 kDa Exposure time: 27 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32315) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")