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![Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32688) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-2882024142262.jpg "Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32688) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32688) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-29820241426269.jpg)
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Immunocytochemistry analysis of HeLa cells labeling p16INK4a/CDKN2A with Mouse anti-p16INK4a/CDKN2AINK4a/CDKN2A antibody (NBP3-32688) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-29202410334055.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (S04-8C9) [NBP3-14885] - Western blot detection of p16INK4a/CDKN2A in 3T3, Hela cell lysates using p16INK4a/CDKN2A Rabbit mAb (1:1000 diluted). Predicted band size: 17KDa. Observed band size: 17KDa.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-S04-8C9-Western-Blot-NBP3-14885-img0002.jpg "Western Blot: p16INK4a/CDKN2A Antibody (S04-8C9) [NBP3-14885] - Western blot detection of p16INK4a/CDKN2A in 3T3, Hela cell lysates using p16INK4a/CDKN2A Rabbit mAb (1:1000 diluted). Predicted band size: 17KDa. Observed band size: 17KDa.")
![Western Blot: p16INK4a/CDKN2A Antibody (S04-8C9) [NBP3-14885] - Western blot detection of p16INK4a/CDKN2A in 293 cell lysates using p16INK4a/CDKN2A Rabbit mAb (1:1000 diluted). Predicted band size: 17KDa. Observed band size: 17KDa.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-S04-8C9-Western-Blot-NBP3-14885-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: p16INK4a/CDKN2A Antibody (S04-8C9) [NBP3-14885] - Immunofluorescence of p16INK4a/CDKN2A (green) in Hela cells using NBP3-14885 at dilution 1/5, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-14885_rabbit-p16ink4a-cdkn2a-mab-s04-8c9-11320251514548.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody [NBP2-98881] - Anti-p16INK4a/CDKN2A rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 17 kDa](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP2-98881-img0005.jpg "Western Blot: p16INK4a/CDKN2A Antibody [NBP2-98881] - Anti-p16INK4a/CDKN2A rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 17 kDa")
![Immunocytochemistry/Immunofluorescence: p16INK4a/CDKN2A Antibody [NBP2-98881] - Immunofluorescence staining of p16INK4a/CDKN2A in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human p16INK4a/CDKN2A polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98881-img0002.jpg)
![Immunoprecipitation: p16INK4a/CDKN2A Antibody [NBP2-98881] - p16INK4a/CDKN2A was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate 2 ul anti-p16INK4a/CDKN2A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-p16INK4a/CDKN2A rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 17 kDa](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Immunoprecipitation-NBP2-98881-img0007.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32689] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Rabbit anti-p16INK4a/CDKN2A antibody (NBP3-32689) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32689) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32689_rabbit-p16ink4a-cdkn2a-mab-pd01-16-278202416331268.jpg "Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32689] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Rabbit anti-p16INK4a/CDKN2A antibody (NBP3-32689) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32689) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32689] - Immunohistochemical analysis of paraffin-embedded human ovary carcinoma tissue with Rabbit anti-p16INK4a/CDKN2A antibody (NBP3-32689) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32689) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32689_rabbit-p16ink4a-cdkn2a-mab-pd01-16-298202414333625.jpg)
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32689] - Immunocytochemistry analysis of SKOV-3 cells labeling p16INK4a/CDKN2A with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32689) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32689) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32689_rabbit-p16ink4a-cdkn2a-mab-pd01-16-29202410484819.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (CDKN2A/4844R) [NBP3-13833] - Western blot analysis of HepG2 cell lysate using p16INK4a/CDKN2A antibody (CDKN2A/4844R).](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-CDKN2A-4844R-Western-Blot-NBP3-13833-img0001.jpg "Western Blot: p16INK4a/CDKN2A Antibody (CDKN2A/4844R) [NBP3-13833] - Western blot analysis of HepG2 cell lysate using p16INK4a/CDKN2A antibody (CDKN2A/4844R).")
![Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody (CDKN2A/4844R) [NBP3-13833] - Formalin-fixed, paraffin-embedded human cervix stained with p16INK4a/CDKN2A antibody (CDKN2A/4844R).](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-CDKN2A-4844R-Immunohistochemistry-Paraffin-NBP3-13833-img0002.jpg)
![Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody (CDKN2A/4844R) [NBP3-13833] - Formalin-fixed, paraffin-embedded human cervix stained with p16INK4a/CDKN2A antibody (CDKN2A/4844R).](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-CDKN2A-4844R-Immunohistochemistry-Paraffin-NBP3-13833-img0003.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (RM409) [NBP2-89102] - Western Blot of 293 cell lysate using Anti-p16 rabbit monoclonal antibody (Clone RM409) at a 1:2000 dilution.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-RM409-Western-Blot-NBP2-89102-img0002.jpg "Western Blot: p16INK4a/CDKN2A Antibody (RM409) [NBP2-89102] - Western Blot of 293 cell lysate using Anti-p16 rabbit monoclonal antibody (Clone RM409) at a 1:2000 dilution.")
![Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody (RM409) [NBP2-89102] - Immunohistochemical staining of formalin fixed and paraffin embedded human cervical squamous carcinoma tissue section using Anti-p16 Rabbit Monoclonal Antibody (Clone RM409) at a 1:200 dilution.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-RM409-Immunohistochemistry-Paraffin-NBP2-89102-img0001.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (RM267) [NBP3-18535] - Western Blot of HeLa cells lysates using NBP3-18535 at a 1:500 dilution.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-RM267-Western-Blot-NBP3-18535-img0001.jpg "Western Blot: p16INK4a/CDKN2A Antibody (RM267) [NBP3-18535] - Western Blot of HeLa cells lysates using NBP3-18535 at a 1:500 dilution.")
![Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody (RM267) [NBP3-18535] - Immunohistochemical staining of formalin fixed and paraffin embedded human colon cancer tissue sections using NBP3-18535 at a 1:1000 dilution.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-RM267-Immunohistochemistry-Paraffin-NBP3-18535-img0002.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (GT1249) [NBP3-13669] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with p16INK4a/CDKN2A antibody [GT1249] (NBP3-13669) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-GT1249-Western-Blot-NBP3-13669-img0002.jpg "Western Blot: p16INK4a/CDKN2A Antibody (GT1249) [NBP3-13669] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with p16INK4a/CDKN2A antibody [GT1249] (NBP3-13669) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody (GT1249) [NBP3-13669] - p16INK4a/CDKN2A antibody [GT1249] detects p16INK4a/CDKN2A protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast. p16INK4a/CDKN2A stained by p16INK4a/CDKN2A antibody [GT1249] (NBP3-13669) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-GT1249-Immunohistochemistry-Paraffin-NBP3-13669-img0001.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (GT888) [NBP3-13670] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with p16INK4a/CDKN2A antibody [888] (NBP3-13670) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-GT888-Western-Blot-NBP3-13670-img0002.jpg "Western Blot: p16INK4a/CDKN2A Antibody (GT888) [NBP3-13670] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with p16INK4a/CDKN2A antibody [888] (NBP3-13670) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody (GT888) [NBP3-13670] - Human breast. p16INK4a/CDKN2A stained by p16INK4a/CDKN2A antibody [888] (NBP3-13670) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-GT888-Immunohistochemistry-Paraffin-NBP3-13670-img0001.jpg)
![Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody (CDKN2A/4499) - Azide and BSA Free [NBP3-08438] - p16-positive head and neck squamous cell carcinoma (HNSCC) stained for P16INK4a. Image from verified customer review](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-CDKN2A-4499-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08438-img0003.jpg "Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody (CDKN2A/4499) - Azide and BSA Free [NBP3-08438] - p16-positive head and neck squamous cell carcinoma (HNSCC) stained for P16INK4a. Image from verified customer review")
![Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody (CDKN2A/4499) - Azide and BSA Free [NBP3-08438] - Formalin-fixed, paraffin-embedded human cervix stained with p16INK4a/CDKN2A Mouse Monoclonal Antibody (CDKN2A/4499).](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-CDKN2A-4499-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08438-img0002.jpg)
![Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody (CDKN2A/4499) - Azide and BSA Free [NBP3-08438] - Formalin-fixed, paraffin-embedded human cervix stained with p16INK4a/CDKN2A Mouse Monoclonal Antibody (CDKN2A/4499).](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-CDKN2A-4499-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08438-img0001.jpg)