Measuring Extracellular Vesicles

John Nolan, Ph.D
Principal Investigator, Nolan Lab

Workshop abstract:
Extracellular vesicles (EVs) are small (~100 nm) membrane-bound vesicles released from the cell surface and intracellular compartments of all cells. EVs are of great interest as clinical biomarkers due to their abundance in blood, CSF, and other body fluids and because they carry protein and nucleic acids from their cell of origin. However, their small size and heterogeneity in biofluids make EVs very challenging to measure. This workshop will highlight the key features of EVs, the fundemental principles underlying their measurement by flow cytometry, and the appropriate calibrators and standards for EV measurements by flow cytometry. Finally, we will introduce a new high resolution flow cytometry approach to EV measurement using a custom high sensitivity flow cytometer and nanoscale calibration and reference particles.

About John Nolan
John Nolan is a Professor at The Scintillon Institute, where his research group develops and applies new technologies for cytometry, including the development of high resolution analysis of extracellular vesicles. Other projects in his lab include spectral flow cytometry, single particle analysis of natural and synthetic nanoparticles, and surface enhanced Raman scattering (SERS) in cytometry. He is on the Editorial Boards of Cytometry and Current Protocols in Cytometry, a Fellow of the American Institute of Medical and Biological Engineering (AIMBE), and Past-president of the International Society for Advancement of Cytometry (ISAC).

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