DY998) followed by chemiluminescent detection using WesternGlo Chemiluminescent Detection Substrate (Catalog # AR004). Human FGF R2 alpha can be detected by the Human Total-FGF R2 alpha DuoSet IC ELISA by using approximately 20 to 40 times less lysate than is needed for a conventional IP-Western blot. " alt="Lysates prepared from the human stomach cancer cell line (KatoIII) were diluted in series and analyzed by (A) IP-Western blot and (B) this DuoSet IC ELISA. IPs were performed using an anti-FGF R2 alpha monoclonal antibody and goat anti-mouse agarose. Immunoblots were incubated with a biotinylated anti-FGF R2 alpha monoclonal antibody. Bands were visualized with Streptavidin-HRP (Catalog # DY998) followed by chemiluminescent detection using WesternGlo Chemiluminescent Detection Substrate (Catalog # AR004). Human FGF R2 alpha can be detected by the Human Total-FGF R2 alpha DuoSet IC ELISA by using approximately 20 to 40 times less lysate than is needed for a conventional IP-Western blot. " class="big_thumb" />
