Tumor-derived Exosome Products

Description

Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both the amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, and virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (broncho alveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases. Novus kit is a patented double sandwich enzyme-linked immunoassay for quantitative and qualitative analysis of exosomes. It is a successful platform for exosomes quantification and characterization from small amount of human biological fluids (plasma, serum, urine, saliva) or cell media. It may be exploited to identify exosomes released by cancer cells in the plasma and urine of tumor patients in various disease conditions. It consists of ELISA plates pre-coated with proprietary pan-exosome antibodies enabling specific capture of exosomes from different biological samples, including cell culture supernatants and human biological fluids. Tumor-derived Exosome enrichment and Quantification Assay Kit includes an immunoplate able to enrich the tumor exosome subpopulation and is especially suitable for biomarker discovery in clinical cancer research or in development of diagnostics. Quantification and characterization of exosomal proteins is subsequently performed using appropriate detection antibodies against exosome associated antigens that can be for either generic or cell/ tissue-specific exosomes. The kit contains Lyophilized Exosome Standards for assay calibration. These standards, which are characterized for protein content and particle number, allow the quantification of unknown sample through a standard calibration curve.

Bioinformatics

Alternate Names

Cancer exosome Cancer-derived exosomes EV detection exosome detection Exosome enrichment Exosome kit Exosome quantification Exosome Exosomes ELISA extracellular vesicle detection Kit for exosomes