Description
Rainbow Calibration Particles are used to ascertain the relative voltage range for each flow cytometer detector. This mixture of fluorescent particles serves as a reliable and reproducible means of evaluating the sensitivity and resolution of flow cytometers.
The relative number of fluorophores can been determined for every emission peak of the rainbow calibration particles in FL1 (FITC, MEFL), FL2 (RPE, MEPE), FL3 (RPE-Cy5, MEPCY) and FL4 (APC, MEAP) channels in order to plot the calibration graph. The calibration graph is used to check the linearity (dynamic range) of the photomultiplier tube (PMT) in each channel. Additionally, the relative number of fluorophores can be cross calibrated with cells or particles stained with a known number of spectral matching fluorophores such as FITC, PE, RPE-Cy5 to estimate the number of fluorophores on stained cells.
Rainbow Calibration Particle Set (8 peaks - EAL01) are used in studies following EuroFlow(TM) standard guidelines for PMT characterization, to set target mean fluorescence intensities (MFI) values and for daily performance tracking of flow cytometers. They provide target MFIs for the standardization of an 8-color flow cytometric immunophenotyping panel of normal and malignant leukocytes in bone marrow and blood (1).
References
1. Kalina, T., Flores-Montero, J., van der Velden V.H.J., Martin-Ayuso, M., Bottcher, S., Ritgen, M., Almeida, J., Lhermitte,L., Asnafi, V., Mendonca, A., de Tute, R., Cullen, M., Sedek, L., Vidriales, M.B., Perez, J.J., te Marvelde, J.G., Mejstrikova, E., Hrusak, O., Szczepanski, T., van Dongen, J.J.M., and Orfao A.. (2012). EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols. Leukemia. 26(9):1986-2010. https://doi.org/10.1038/leu.2012.122
EuroFlow(TM) is a trademark of European Scientific foundation for Laboratory Hemato Oncology (ESLHO).
Bioinformatics
| Alternate Names |
- Calibration Beads
- Flow Cytometry Calibration
- Rainbow Calibration Beads
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