Easy R-PE labeling of your antibody with 30 seconds hands-on time. R-PE (R-Phycoerythrin) is a large 240kDa red phycobiliprotein isolated from red algae. R-PE has a strong absorption peak at 565nm and a secondary absorption peak at 492nm. The R-PE protein is commonly used for fluorescent immunolabeling, particularly in applications involving fluorescent-activated cell sorting.
The Lightning-Link labeling system represents a quantum leap forward in antibody labeling technology. It allows the researcher to make labeled antibodies with minimal hands-on time - less than 30 seconds. Lightning-Link simplifies immunoassay techniques, such as western blotting, ELISA and immunohistochemistry, by eliminating secondary reagents and cutting the number of incubation and wash steps.
Despite its simplicity, Lightning-Link is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled fashion, creating high quality labeled antibodies and proteins.
As the procedure is not interrupted by desalting steps, trial labels can be prepared with microgram quantities of protein and then scaled up with ease. Lightning-Link eliminates problems associated with scale up; hands-on time is essentially independent of process scale. Consistency from batch to batch is easy to achieve, and recoveries approach 100%.