Easy PE/Cy5 labeling of your antibody with 30 seconds hands-on time. PE/Cy5 is a tandem conjugate. The PE is excited at 488nm and functions as an energy donor for the Cy5. Energy is transferred from the PE to the Cy5 via energy resonance transfer. The Cy5 emits the energy received from the PE in the form of long wavelength light at 679nm.
The Lightning-Link conjugation system represents a quantum leap forward in conjugation technology. It allows you to make antibody conjugates with minimal hands-on time - less than 30 seconds. Lightning-Link simplifies immunoassay techniques, such as western blotting, ELISA and immunohistochemistry, by eliminating secondary reagents and cutting the number of incubation and wash steps.
Despite its simplicity, Lightning-Link is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled fashion, creating high quality conjugates.
As the procedure is not interrupted by desalting steps, trial conjugates can be prepared with microgram quantities of protein and then scaled up with ease. Lightning-Link eliminates problems associated with scale up; hands-on time is essentially independent of process scale. Consistency from batch to batch is easy to achieve, and recoveries approach 100%.