Description
The Innova FlexLISA kits are designed for the development and optimization of sandwich ELISA assays to detect the presence of any antigen with a high affinity for any antibody pair in complex samples (serum, plasma, urine etc.). At the core of the kit, there is the 30 seconds hands-on time Lightning-Link conjugation technology for the labeling of the capture and detection antibodies of choice and a biotin precoated 96 well stripwell plate provided either clear or black, depending on the enzymatic substrate. The main advantage of this format is that the amount of capture antibody is reduced up to 40-fold, allowing ELISAs to be developed in a cost-effective way using small amounts of commercially available antibodies. The format also allows more than one capture reagent to be used, allowing multiple analytes to be assessed on a single plate. Each FlexLISA kit allows you to conjugate, in 30 seconds handson time, up to 3 different capture antibodies to streptavidin using the 3 x 10ug Lightning-Link-Streptavidin kit, and up to 3 different detection antibodies to either horseradish peroxidase (HRP) or alkaline phosphatase (AP) using the 3 x 10ug LightningLink-HRP or Lightning-Link-AP kits. A rapid one-step incubation protocol is used in this kit, employing pre-blocked microtiter well strips coated with biotin. The FlexLISA assay is run by adding the sample and the antibody mix to the wells, incubating for 1 hour, washing and reading the assay plate. FlexLISA is ideal for quick and reliable ELISA assay development, antibody pair screening and ELISA assay optimization.
