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Flagellin, TLR5 Ligand Products


Description

Flagellin ligand activity on TLR5/NF-kB SEAPorter™ HEK 293 cell line (Cat. No. NBP2-26277 [TLR5 positive; TLR2/TLR4 negative]) and NF-kB SEAPorter™ RAW 264.7 cell line (Cat. No. NBP2-26261 [TLR5 negative; TLR2/TLR4 positive]). NBP2-26277 is a stably co-transfected cell line that expresses full-length human Toll-like receptor 5 (TLR5) and the secreted alkaline phosphatase (SEAP) reporter gene under the transcriptional control of an NF-kB response element. NBP2-26261 is a stably transfected cell line which expresses the NF-kB/SEAP reporter gene. NBP2-26277 and NBP2-26261 cells were plated in 96-well plates at 5 x 10^4 cells/well and 8.5 x 10^4 cells/well, respectively. After 16 h, cells were stimulated with various amounts of Flagellin (NBP2-25289) as well as 500 ng/ml LPS (NBP2-26216) and 50 ng/ml Pam3CSK4 for 24 h. SEAP was analyzed using the SEAPorter Assay Kit (Cat. No. NBP2-25285). Please see figures 1 and 2.