The Antibody concentration and Clean Up Kit allows for the quick and easy concentration of antibodies and proteins. Antibodies are sometimes only available at low concentrations and often contain low molecular weight substances that interfere in labeling reactions with enzymes, biotin, streptavidin and fluorophores. The kit can also be used to reduce the concentration of many unwanted additives often found in antibody formulations such as azide, glycine or tris. The antibody clean up kit method utilizes a simple spin column to easily and quickly remove excess buffer from the antibody thereby providing a more concentrated antibody solution. The clean up kit also allows the experimenter to perform a simple buffer exchange to transfer the antibody into a more favorable conjugation buffer.