Conjugate antibodies

Tips to Optimize Your Western Blot for Phosphorylated Protein Detection

Western blotting allows identifying and quantifying proteins from cell lysates for determination of protein expression, sub-cellular localization, post-translational modifications, protein processing, and protein-protein interactions.

By Jamshed Arslan, Pharm. D., PhD.

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Secondary antibodies

Phosphorylated proteins

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Considerations for Quantitative Western blotting

GAPDH expression in human nucleus pulposus cell lysate, WB

By Jamshed Arslan, Pharm. D., PhD.

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