Flow Cytometry is a laser based biological assay used for cell detection, counting and sorting of specific biomarkers. Cells are first suspended in solution and fluorescently labeled antibodies are applied. Then the cells are passed single-file through a series of lasers which analyze size, shape and the fluorescent characteristics of each cell. Results can then be electronically gated to filter the data to a specific cell type and whether or not they contain bound antibodies. This form of assay is particularly useful for analyzing blood samples.
Novus offers a range of support options for flow cytometry when you buy a product to help you with your flow cytometry experiment including over 1,000 Flow images using our pre-conjugated antibodies:
![Lightning-Link APC-Cy7 Antibody Labeling Kit [765-0030] - Mouse anti-human CD8 was conjugated with APC-Cy7 using an Expedeon Lightning-Link kit. The conjugated antibody was then used to stain human peripheral blood lymphocytes, followed by analysis with flow cytometry. (Blue line - negative control; red line - positive staining).](http://images.novusbio.com/fullsize/Lightning-Link-APC-Cy7-Antibody-Labeling-Kit-765-0030-img0001.jpg "Lightning-Link APC-Cy7 Antibody Labeling Kit [765-0030] - Mouse anti-human CD8 was conjugated with APC-Cy7 using an Expedeon Lightning-Link kit. The conjugated antibody was then used to stain human peripheral blood lymphocytes, followed by analysis with flow cytometry. (Blue line - negative control; red line - positive staining).")
![Flow Cytometry: Lightning-Link PerCP Antibody Labeling Kit [718-0030] - Mouse anti-human CD3 was conjugated with PerCP using a Lightning-Link kit. The conjugated antibody was then used to stain human peripheral blood lymphocytes, followed by analysis with flow cytometry. (Blue line - negative control; red line - positive staining).](http://images.novusbio.com/fullsize/Lightning-Link-PerCP-Antibody-Labeling-Kit-Flow-Cytometry-718-0030-img0001.jpg "Flow Cytometry: Lightning-Link PerCP Antibody Labeling Kit [718-0030] - Mouse anti-human CD3 was conjugated with PerCP using a Lightning-Link kit. The conjugated antibody was then used to stain human peripheral blood lymphocytes, followed by analysis with flow cytometry. (Blue line - negative control; red line - positive staining).")
![Flow Cytometry: Lightning-Link Rapid Atto565 Antibody Labeling Kit [351-0010] - Mouse anti-human CD3 was conjugated with Atto565 using Lightning-Link® Rapid kit. The conjugated antibody was then used to stain human peripheral blood lymphocytes, followed by analysis with flow cytometry. (Blue line - negative control; red line - positive staining).](http://images.novusbio.com/fullsize/Lightning-Link-Rapid-Atto565-Antibody-Labeling-Kit-Flow-Cytometry-351-0010-img0003.jpg "Flow Cytometry: Lightning-Link Rapid Atto565 Antibody Labeling Kit [351-0010] - Mouse anti-human CD3 was conjugated with Atto565 using Lightning-Link® Rapid kit. The conjugated antibody was then used to stain human peripheral blood lymphocytes, followed by analysis with flow cytometry. (Blue line - negative control; red line - positive staining).")
![Lightning-Link Rapid Atto565 Antibody Labeling Kit [351-0010]](http://images.novusbio.com/fullsize/Lightning-Link-Rapid-Atto565-Antibody-Labeling-Kit-351-0010-img0001.jpg)
![Lightning-Link Rapid DyLight 488 Antibody Labeling Kit [322-0005] - Mouse anti-human CD3 was conjugated with DyLight 488 using an Expedeon Rapid Lightning-Link kit. The conjugated antibody was then used to stain human peripheral blood lymphocytes, followed by analysis with flow cytometry. (Blue line - negative control; red line - positive staining).](http://images.novusbio.com/fullsize/Lightning-Link-Rapid-DyLight-488-Antibody-Labeling-Kit-322-0005-img0001.jpg "Lightning-Link Rapid DyLight 488 Antibody Labeling Kit [322-0005] - Mouse anti-human CD3 was conjugated with DyLight 488 using an Expedeon Rapid Lightning-Link kit. The conjugated antibody was then used to stain human peripheral blood lymphocytes, followed by analysis with flow cytometry. (Blue line - negative control; red line - positive staining).")
![Flow Cytometry: Lightning-Link Rapid DyLight 550 Antibody Labeling Kit [323-0010] - Mouse anti-human CD3 was conjugated with Dylight® 550 using Lightning-Link ® Rapid kit. The conjugated antibody was then used to stain human peripheral blood lymphocytes, followed by analysis with flow cytometry. (Blue line - negative control; red line - positive staining).](http://images.novusbio.com/fullsize/Lightning-Link-Rapid-DyLight-550-Antibody-Labeling-Kit-Flow-Cytometry-323-0010-img0003.jpg "Flow Cytometry: Lightning-Link Rapid DyLight 550 Antibody Labeling Kit [323-0010] - Mouse anti-human CD3 was conjugated with Dylight® 550 using Lightning-Link ® Rapid kit. The conjugated antibody was then used to stain human peripheral blood lymphocytes, followed by analysis with flow cytometry. (Blue line - negative control; red line - positive staining).")
![Lightning-Link Rapid DyLight 550 Antibody Labeling Kit [323-0010]](http://images.novusbio.com/fullsize/Lightning-Link-Rapid-DyLight-550-Antibody-Labeling-Kit-323-0010-img0001.jpg)
![Flow Cytometry: Lightning-Link Rapid DyLight 550 Antibody Labeling Kit [323-0030] - Mouse anti-human CD3 was conjugated with Dylight® 550 using Lightning-Link ® Rapid kit. The conjugated antibody was then used to stain human peripheral blood lymphocytes, followed by analysis with flow cytometry. (Blue line - negative control; red line - positive staining).](http://images.novusbio.com/fullsize/Lightning-Link-Rapid-DyLight-550-Antibody-Labeling-Kit-Flow-Cytometry-323-0030-img0002.jpg "Flow Cytometry: Lightning-Link Rapid DyLight 550 Antibody Labeling Kit [323-0030] - Mouse anti-human CD3 was conjugated with Dylight® 550 using Lightning-Link ® Rapid kit. The conjugated antibody was then used to stain human peripheral blood lymphocytes, followed by analysis with flow cytometry. (Blue line - negative control; red line - positive staining).")
![Lightning-Link Rapid DyLight 550 Antibody Labeling Kit [323-0030]](http://images.novusbio.com/fullsize/Lightning-Link-Rapid-DyLight-550-Antibody-Labeling-Kit-323-0030-img0001.jpg)
![Flow Cytometry: Lightning-Link Rapid DyLight 594 Antibody Labeling Kit [324-0030] - Mouse anti-human CD3 was conjugated with Dylight® 594 using Lightning-Link ® Rapid kit. The conjugated antibody was then used to stain human peripheral blood lymphocytes, followed by analysis with flow cytometry. (Blue line - negative control; red line - positive staining).](http://images.novusbio.com/fullsize/Lightning-Link-Rapid-DyLight-594-Antibody-Labeling-Kit-Flow-Cytometry-324-0030-img0002.jpg "Flow Cytometry: Lightning-Link Rapid DyLight 594 Antibody Labeling Kit [324-0030] - Mouse anti-human CD3 was conjugated with Dylight® 594 using Lightning-Link ® Rapid kit. The conjugated antibody was then used to stain human peripheral blood lymphocytes, followed by analysis with flow cytometry. (Blue line - negative control; red line - positive staining).")
![Lightning-Link Rapid DyLight 594 Antibody Labeling Kit [324-0030]](http://images.novusbio.com/fullsize/Lightning-Link-Rapid-DyLight-594-Antibody-Labeling-Kit-324-0030-img0001.jpg)
![Flow Cytometry: Lightning-Link Rapid DyLight 633 Antibody Labeling Kit [325-0010] - Mouse anti-human CD3 was conjugated with Dylight® 633 using an Innova Biosciences Rapid Lightning-Link® kit. The conjugated antibody was then used to stain human peripheral blood lymphocytes, followed by analysis with flow cytometry. (Blue line - negative control; red line - positive staining).](http://images.novusbio.com/fullsize/Lightning-Link-Rapid-DyLight-633-Antibody-Labeling-Kit-Flow-Cytometry-325-0010-img0004.jpg "Flow Cytometry: Lightning-Link Rapid DyLight 633 Antibody Labeling Kit [325-0010] - Mouse anti-human CD3 was conjugated with Dylight® 633 using an Innova Biosciences Rapid Lightning-Link® kit. The conjugated antibody was then used to stain human peripheral blood lymphocytes, followed by analysis with flow cytometry. (Blue line - negative control; red line - positive staining).")
![Flow Cytometry: Lightning-Link PE-Texas Red Antibody Labeling Kit [767-0015]](http://images.novusbio.com/fullsize/Lightning-Link-PE-Texas-Red-Antibody-Labeling-Kit-Flow-Cytometry-767-0015-img0002.jpg "Flow Cytometry: Lightning-Link PE-Texas Red Antibody Labeling Kit [767-0015]")
![Flow Cytometry: Lightning-Link APC-Cy5.5 Antibody Labeling Kit [764-0010] - APC/Cy5.5 is a tandem conjugate. The APC is excited at 633nm and functions as an energy donor for the Cy5.5. Energy is transferred from the APC to the Cy5.5 via FRET (Fluorescence Resonance Energy Transfer). The Cy5.5 emits then the energy received from the APC in the form of long wavelength light at 694nm.](http://images.novusbio.com/fullsize/Lightning-Link-APC-Cy5.5-Antibody-Labeling-Kit-Flow-Cytometry-764-0010-img0001.jpg "Flow Cytometry: Lightning-Link APC-Cy5.5 Antibody Labeling Kit [764-0010] - APC/Cy5.5 is a tandem conjugate. The APC is excited at 633nm and functions as an energy donor for the Cy5.5. Energy is transferred from the APC to the Cy5.5 via FRET (Fluorescence Resonance Energy Transfer). The Cy5.5 emits then the energy received from the APC in the form of long wavelength light at 694nm.")