AKT1 and AKT2 Pathway Assay Kit Summary
| Description |
This ELISA product range provides a quick, easy, more sensitive, and qualitatively better alternative to western blotting for kinase assay readouts, and it is designed to demonstrate the qualitative differences in the amounts of specific target proteins between samples. |
| Specificity |
This Assay kit contains (1) 96 well strip plate containing AKT1 (pS473), AKT2 (pS474), 3 pairs of strips each (12 strips total) for the qualitative detection of phosphorylated Akt1 and Akt2 in tissue lysates, cell lysates and recombinant protein preparationsas as well as positive controls. |
| Kit Type |
Pathway Assay Kit |
Applications/Dilutions
| Dilutions |
- ELISA
- Immunoassay
- In vitro assay
|
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze. |
Kit Components
|
Components
|
- 2 x 8-microwell strips
- 20x Wash buffer Concentrate
- 5x Sample Dilution Buffer Concentrate
- Antibody-Coated Microwells
- Assay Control
- Biotin-Conjugated Detection Antibody
- Microwell Strip Holder
- Plate Cover
- Stop Solution
- Streptavidin Horseradish Peroxidase
- Tetramethylbenzidine Substrate
|
Notes
You may also Mix-and-Match you own Pathway Assay Kit by purchasing catalog number NBP1-71668. Choose up to 6 different targets from this list: Active Beta Catenin (DP S33/S37/T41), AKT1 (pS473), AKT2 (pS474), CREB (pS133), ERK1/2 (pT202/Y204; pT185/Y187), GSK3 alpha (pS21), GSK3 beta (pS9), p38 (pT180/Y182).
View All Available Pathway Assay Kits Background
Multi-kinase arrays are an alternative analysis platform to traditional Western blots. Novus' Pathway Assay Kits allow for the simultaneous detection of activation states of multiple kinases in a signal pathway on a single multiwell platform. These kits are easier and faster than traditional Western blots (2.5 hour protocol), demonstrate a significantly greater dynamic range and sensitivity, operate with much less sample (5-20 micrograms total protein), and are more cost-effective with up to six data points per assay.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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