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Human, Mouse, Rat Mix-and-Match Pathway Assay ELISA Kit Summary
Specificity
The Novus Mix-and-Match Pathway Assay Kit is designed to simultaneously run assays for multiple kinases on a single plate.
Kit Type
ELISA Kit
Applications/Dilutions
Dilutions
ELISA
Immunohistochemistry
Immunohistochemistry-Paraffin
Application Notes
Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24112408)
For the qualitative detection of differences in the levels of active phospho-proteins in key cell signaling pathways in tissue lysates, cell lysates and recombinant protein preparations. Novus Pathway Assay kits provide a quick, easy, more sensitive, and qualitatively better alternative to western blotting for kinase assay readouts.
The flexibility of the Mix and Match multi-kinase ELISA array option gives researchers more of the data that they want, faster and better than any single analyte competitor system available today.
Simply choose 6 of the 8 analytes below in any combination. Each selection consists of 1 pair of 8-well Antibody-Coated Microwell strips (16 tests), together with its complementary Detection Antibody.
1. p-p38 alpha (T180/Y182)
2. p-ERK2 (T183/Y185)/p-ERK1 (T202/Y204)
3. pCREB (S133)
4. p-AKT1 (S473)
5. p-AKT2 (S474)
6. Beta-Catenin (DPS33/37/T41)
7. p-GSK alpha (S21)
8. p-GSK Beta (S9)
href="http://www.novusbio.com/Pathway-Assay-Kits" target="_blank">View All Available Pathway Assay Kits
Reviewed Applications
Read 1 Review rated 4 using NBP1-71668 in the following applications:
Multi-kinase arrays are an alternative analysis platform to traditional Western blots. Novus' Pathway Assay Kits allow for the simultaneous detection of activation states of multiple kinases in a signal pathway on a single multiwell platform. These kits are easier and faster than traditional Western blots (2.5 hour protocol), demonstrate a significantly greater dynamic range and sensitivity, operate with much less sample (5-20 micrograms total protein), and are more cost-effective with up to six data points per assay.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
Publications for Mix-and-Match Pathway Assay ELISA Kit (NBP1-71668)(2)
We have publications tested in 1 confirmed species: Human.
We have publications tested in 2 applications: IHC-P, SDS-Page.
FAQs for Mix-and-Match Pathway Assay ELISA Kit (NBP1-71668). (Showing 1 - 1 of 1 FAQs).
What is the difference between the Quantikine and Duoset ELISA kits?
Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
