ATG5 Antibody (NBP1-28461)

ATG5 Antibody

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ATG5 Antibody Summary:
Applications:IHC, PEP-ELISA
Clonality:Polyclonal
Gene:ATG5
Purity:Immunogen affinity purified
Host:Goat
 
Publications:Antibody has at least 1 Background Reference.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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ATG5 Antibody Details:
Isotype:IgG
Immunogen:Peptide with sequence SQVINEMQKKDHKQ from the internal region according to NP_004840.1
Epitope:C terminus
Species Reactivity:

Human, Mouse, Cow, Horse

Applications:
Uses:Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Preliminary experiments gave an approx. 38 kDa band in NIH3T3 lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.4 kDa according to NP_004840.1. The 38 kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Dilutions:Immunohistochemistry 1:200, Peptide ELISA 1:16000
Unit Size:0.1 mg
Concentration:0.5 mg/ml
Notes:
Please note, this antibody is considered “Innovators Grade”. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Under this program, Novus will provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal value in exchange for your new data. Please contact us at innovators@novusbio.com for more details.
Packaging:
Storage:Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Buffer:Tris-saline [pH7.3] with 0.5% BSA
Preservative:0.02% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: ATG5
Entrez9474 (Human)
11793 (Mouse)
Swiss ProtNP_004840.1 (Human)
 
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Jump to: Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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