Apoptosis is a programmed cell death mechanism characterized by depolymerization of cytoskeleton, cell shrinkage, chromatin condensation, nuclear fragmentation and translocation of phosphatidylserine to the cell surface. Apoptosis arises from a number of stimuli that initiate multiple signaling pathways which lead to caspase cascade activation and cell death. Apoptosis is a normal series of events and advantageous to organisms, as it prevents damaged cells from consuming essential nutrients and spreading infection. Increases in apoptotic activity are hallmarks of several disease states including AIDS, neurodegenerative disorders, insulin-dependent diabetes, myocardial infarction and atherosclerosis.
Novus Biologicals offers a variety of apoptosis research tools such as proteins and antibodies for caspases, p53 pathway, death receptor pathway, mitochondria-mediated pathway, and various apoptosis inhibitors, inducers, regulators, and apoptosis detection kits etc.
To request an Apoptosis catalog or join our mailing list, please fill out the catalog request form here.
Browse our apoptosis antibodies including:
All Apoptosis Antibodies, Lysates, Proteins and RNAi
Novus Cell Death Research Tools
||Cell impermeant nuclear stain. Will only enter dead/dying cells with compromised membranes and fluoresce (Commonly excited by the UV laser or weakly by the 405nm violet laser and emits at 455nm)
||Cell impermeant nuclear stain. Will only enter dead/dying cells with compromised membranes and fluoresce (Commonly excited by the 488nm blue laser and emits in FL2. Not compatible with PE)
||Cell permeant dye that is cleaved by esterases and fluoresces in live but not dead cells. (Excited by the 488nm blue laser and emits in FL1)
|Calcein Blue AM
||Mechanistically identical to Calcein AM but with different spectral properties (Excited by the UV laser and emits at 450nm similarly to DyLight 350)
|Annexin Based Reagents
|Bind phosophatidylserine (PS) which is externalized in apoptotic cells.
||Binds PS irreversibly. Available in multiple formats (APC, FITC, Biotin) for multicolor flow panels.
||Binds PS reversibly and fluoresces in FL1 when bound. Allows for real-time tracking of apoptosis and detection of the reversibility of early steps in the apoptotic cascade.
View the Novus Biological's real-time tracking of cell death (pSIVA) Webinar.
Antibodies for cancer and apoptosis research
Research products such as Akt antibodies and Actin antibodies are widely used in cancer research. Techniques such as immunoprecipitation and paraffin-embedded immunohistochemistry are used to track and detect proteins associated with apoptosis (programmed cell death) and tumor formation.
We, at Novus Biologicals, supply a vast range of antibodies for the study of cancer and apoptosis. Whether you need an Actin, Bax or Caspase 3 antibody, we can supply highly specific, affordable products across the full range of apoptosis targets.
Actin is found in all eukaryotic cells, in three main isoforms - alpha, beta and gamma. Each Actin isoform is specific to a certain cell type and performs distinct functions. In addition, we provide specific Actin antibodies for each isoform.
Beta-actin is found in all mammalian cells and, thus, is widely used in Western blot detection assays as a loading control. A specific beta-actin antibody is then employed to study its distribution. Beta-actin antibodies are of particular importance in cancer research, as raised levels of Beta-actin have been implicated in the formation of breast tumor cells.
, also known as Protein Kinase B (PKB), acts to inhibit apoptosis and to promote proliferation. Akt antibody formation has been noted in active cancer cells, where it inhibits the action of Akt. Akt genes are heavily implicated in normal human apoptosis, meaning excess Akt antibody production can lead to tumor formation.
Akt production can be triggered by clinical stimuli such as chemotherapy, so Akt antibody preparations are also used to monitor the affects of anti-cancer drugs.
Bax antibodies are used as markers for Bax, a protein which promotes apoptosis, and is heavily implicated in the growth of tumor cells.
Researchers determine which Bax antibodies to use based on which applications they are using for Bcl-2 Antibodies
Bcl-2 antibodies, similar to Bax antibodies, are used in apoptosis research. There are at least 25 Bcl-2 proteins on the market, implicated in a large number of cancers - our Bcl-2 antibody range is similarly extensive.
Bcl-2 refers to a specific lymphoma protein. Monoclonal Bcl-2 antibody is used to detect both Bcl-2 and prevent its pro-apoptotic action.
Caspase 3 antibodies
Capsase 3 antibodies are used to study Caspase 3 cleavages - an important element of apoptosis. Capsase 3 antibodies are used to detect both full-length and cleaved Caspase 3 proteins.
Capsase 3 antibodies are also used in Alzheimer's research, as Caspase 3 levels are linked to neuronal death.
Survivin antibody preparations are widely used in apoptosis research, predominantly to detect Survivin in adult tissues. Survivin is switched off during fetal development; therefore, levels of Survivin in healthy cells will be close to zero. In tumor cells, Survivin reactivates; Survivin antibodies will detect these elevated levels.
Survivin antibodies detect both nuclear and cytoplasmic Survivin localization in a wide range of applications.
Our antibody catalog covers all areas of cancer research, apoptosis research and many other segments of scientific exploration. Our products contain full technical data and are guaranteed to work in the applications and species stated on the datasheet. We have a range of antibody targets to suit your needs and research budget.