CD40/TNFRSF5 Antibody (5C3) - BSA Free

Flow Cytometry: CD40/TNFRSF5 Antibody (5C3) [NBP1-43416] - Analysis using the PE conjugate of NBP1-43416. Analysis of human peripheral blood leukocytes using CD40 PE conjugate. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8 cells/test.

Flow Cytometry: CD40/TNFRSF5 Antibody (5C3) [NBP1-43416] - Analysis using the FITC conjugate of NBP1-43416. Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control FITC (blue histogram) or Anti-Human CD40 FITC (purple histogram). Cells in the lymphocyte gate were used for analysis.

• Reactivity: Hu, Mu
• Applications: Flow, ICC/IF, IHC
• Clone: 5C3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

CD40/TNFRSF5 Antibody (5C3) - BSA Free Summary

• Description: Novus Biologicals Mouse CD40/TNFRSF5 Antibody (5C3) - BSA Free (NBP1-43416) is a monoclonal antibody validated for use in IHC, Flow and ICC/IF. Anti-CD40/TNFRSF5 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Clone 5C3 was used by HLDA to establish CD designation.
• Immunogen: The immunogen for this antibody was CD40.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD40
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
• Application Notes: The 5C3 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. This antibody may also be used for immunohistochemical staining of frozen tissue sections. 5C3 has also been reported in in vitro functional studies.

Reactivity Notes

Mouse reactivity reported in scientific literature (Chechneva O et al).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD40/TNFRSF5 Antibody (5C3) - BSA Free

• B cell surface antigen CD40
• B-cell surface antigen CD40
• Bp50B cell-associated molecule
• CD40 antigen
• CD40 molecule, TNF receptor superfamily member 5
• CD40 type II isoform
• CD40
• CD40L receptor
• CDw40
• MGC9013
• nerve growth factor receptor-related B-lymphocyte activation molecule
• p50
• TNFRSF5
• TNFRSF5CD40 antigen (TNF receptor superfamily member 5)
• tumor necrosis factor receptor superfamily member 5
• tumor necrosis factor receptor superfamily, member 5

Background

The 5C3 monoclonal antibody reacts with human CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development and maturation by inducing Ig isotype-switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation. 5C3 is reported to be used for activation of B cells in in vitro functional assays.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD40/TNFRSF5 Antibody (NBP1-43416)(2)

Publications using NBP1-43416

• Chechneva O, Mayrhofer F, Dariychuk Z et al. p38a in CD11c+cells drives sex-specific differences during progression of autoimmune demyelination bioRxiv 2018-08-20 (IF/IHC, Mouse)
• Woodruff M, Ramonell R, Cashman K et al. Critically ill SARS-CoV-2 patients display lupus-like hallmarks of extrafollicular B cell activation medRxiv 2020-05-03 [PMID: 32511635]

FAQs for CD40/TNFRSF5 Antibody (NBP1-43416). (Showing 1 - 2 of 2 FAQs).

1. I wanna know does CD40 antibody for IHC-P comes with positive control slide?
   • Our CD40 antibodies do not come with positive control slides however you can use any type of primary carcinoma cell as a positive control. I recommend breast carcinoma as this is what we have tested our CD40 antibodies on.
2. We are studying CD40L in our system. It has been known that both CD40 and CD11b are receptors for CD40L. We need CD40 antibody to neutralize CD40 binding to CD40L, then we may prove what is function for CD11b binding to CD40L. Do you have any CD40 Ab which has block function only between CD40 and CD40L, but not between CD11b and CD40L?
   • Unfortunately, none of our CD40 antibodies have been specifically tested for this application.

Bioinformatics

• Gene Symbol: CD40