The ATG5 protein belongs to the ATG autophagy regulator family. This family controls the highly conserved cell’s homeostatic response to a wide variety of both self- and foreign-originating cellular stimuli. ATG5 itself is ubiquitously expressed in most cells and most often found co-localized with the cytoplasmic non-muscle actin protein under normal resting conditions. Upon activation of apoptosis, ATG5 expression is then dramatically intensified, with ATG5 directly complexing with its ATG family members to produce autophagosomes. An intriguing application of the ATG5 antibody was done by Herd’s group where they used it to characterize the effect of silica nanomaterials on both cell uptake and toxicity in epithelial phagocytic cells1. The antibody allowed this group to monitor the effect of physicochemical manipulation of these silica-based nanoparticles on lysosomal formation and function, autophagic like activity, and compartmental fusion and recycling.
A protective role for autophagy in chronic obstructive pulmonary disease (COPD) in response to tobacco smoke was identified by for Fujii et al who performed ATG5 antibody immunoblotting as an autophagy marker2. Kang’s group used the ATG5 antibody in their studies linking autophagy to interleukin 2 (IL2)-induced cell growth, apoptosis, and immmunoregulation – a novel link for autophagy in survival regulation and wound healing3. A recent Nature Communication publication highlights the use of the ATG5 antibody to demonstrate an unconventional function of ATG proteins such as ATG5 and ATG7 as molecular scaffolds in the ubiquitous extracellular signal-regulated kinase (ERK) signal cascade4. Yen’s group used the ATG5 antibody in both immunoblotting and immunohistochemistry experiments examining the effects on autophagy modification with drugs such as rapamycin and activated protein C (APC) in treating and repairing sepsis-induced acute lung injury5.
Novus Biologicals offers ATG5 reagents for your research needs including: