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PGC-1 beta Antibody [Biotin]

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Product Details

Summary
Product Discontinued
View other related PGC-1 beta Primary Antibodies

Order Details


    • Catalog Number
      NB110-58858B
    • Availability
      Product Discontinued

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PGC-1 beta Antibody [Biotin] Summary

Immunogen
A recombinant protein made to the N-terminal region of the human PGC-1 beta protein (within residues 1-500) [UniProt Q86YN6].
Localization
Nuclear (may also see some cytoplasmic localization).
Clonality
Polyclonal
Host
Rabbit
Gene
PPARGC1B
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
This PGC-1 beta antibody can be used for Immunohistochemistry on paraffin embedded sections and Western blot. In Western blot a band is observed at ~113 kDa in endogenous samples (~44 kDa in recombinant protein). This antibody has been tested endogenously in Western blot against human skeletal muscle, HeLa nuclear extracts, A673 and DU145 whole cell extracts, demonstrating the correct band but also giving many non-specific bands. A true negative control is strongly recommended to determine the exact band of interest in your positive sample. It has also been used for IHC-P in human cortical neurons.

Reactivity Notes

Reacts with human and mouse. 76% sequence identity with mouse and rat. 82% sequence identity with equine. 73% sequence identity with canine.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PGC-1 beta Antibody [Biotin]

  • DKFZp686C1790
  • ERRL1
  • PERCFLJ14284
  • peroxisome proliferator-activated receptor gamma, coactivator 1 beta
  • PGC1
  • PGC1Bgamma, coactivator 1, beta
  • PPARGC1

Background

PGC-1 beta is a tissue specific co-activator that enhances the activity of estrogen receptor alpha. The co-activation function relies on a bipartite transcriptional activation domain and two LXXLL motifs that interact with the AR2 domain of ER-alpha in an estrogen-dependent manner. Ectopic expression of this protein has been shown to lead to increased mitochondrial number and basal oxygen consumption.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PPARGC1B