Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
Synthetic peptide corresponding to residues on the C terminus of human LCAT.
Species Reactivity:
Reacts in human. Does not react in mouse or rat.
Applications:
Uses:
This antibody is useful for flow cytometry / FACS analysis, Immunocytochemistry, Immunohistochemistry-Paraffin, immunoprecipitation and Western Blot
Dilutions:
Flow Cytometry 1:10, Immunocytochemistry 1:100 - 250, Immunohistochemistry-Paraffin 1:100 - 250, immunoprecipitation 1:100, Western Blot 1:10000
Unit Size:
0.1 ml
Concentration:
This product is unpurified. Concentration is not relevant.
Packaging:
Storage:
Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Buffer:
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA.
Preservative:
0.01% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
The enzyme, lecithin cholesterol acyltransferase (LCAT), is responsible for the esterification of plasma cholesterol mediating the transfer of an acyl group from lecithin to the 3-hydroxy group of cholesterol (1). Esterification of cholesterol in plasma is mediated by LCAT. In patients with familial LCAT deficiency only trace amounts or no LCAT protein is found. Heterozygotes for this disorder have approximately half the normal amount of the enzyme. LCAT reactivity is essential for normal lipoprotein metabolism and for a proper equilibrium between tissue and plasma cholesterol (2). Cholesterol transport in circulation and its removal from tissues depends on the activity of lecithin cholesterol LCAT. LCAT is a soluble enzyme that converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lyso-phosphatidylcholines on the surface of high-density lipoproteins (3).