LCAT Antibody (EPR1384Y) Summary
| Immunogen |
Synthetic peptide corresponding to residues on the C terminus of human LCAT. |
| Specificity |
A synthetic peptide corresponding to residues on the C-terminus of human LCAT was used as an immunogen. |
| Isotype |
IgG |
| Clonality |
Monoclonal |
| Host |
Rabbit |
| Gene |
LCAT |
| Purity |
Tissue culture supernatant |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- Flow Cytometry 1:10
- Immunocytochemistry/ Immunofluorescence 1:100 - 250
- Immunohistochemistry
- Immunohistochemistry-Paraffin 1:100 - 250
- Immunoprecipitation 1:100
- Western Blot 1:10000
|
| Application Notes |
This antibody is useful for flow cytometry / FACS analysis, Immunocytochemistry, Immunohistochemistry-Paraffin, immunoprecipitation and Western Blot |
| Publications |
|
Reactivity Notes
Reacts with: Human Does not react with Mouse, Rat
Packaging, Storage & Formulations
| Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer |
49% PBS, 0.05% BSA and 50% Glycerol |
| Preservative |
0.01% Sodium Azide |
| Purity |
Tissue culture supernatant |
Notes
Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Alternate Names for LCAT Antibody (EPR1384Y)
Background
The enzyme, lecithin cholesterol acyltransferase (LCAT), is responsible for the esterification of plasma cholesterol mediating the transfer of an acyl group from lecithin to the 3-hydroxy group of cholesterol (1). Esterification of cholesterol in plasma is mediated by LCAT. In patients with familial LCAT deficiency only trace amounts or no LCAT protein is found. Heterozygotes for this disorder have approximately half the normal amount of the enzyme. LCAT reactivity is essential for normal lipoprotein metabolism and for a proper equilibrium between tissue and plasma cholesterol (2). Cholesterol transport in circulation and its removal from tissues depends on the activity of lecithin cholesterol LCAT. LCAT is a soluble enzyme that converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lyso-phosphatidylcholines on the surface of high-density lipoproteins (3).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Publications for LCAT Antibody (NB110-57158)(1)
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Product General Protocols
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