Insulin Receptor Antibody (NB400-142)
Insulin Receptor Antibody (MA-20)
|
This product is fully covered under the Novus guarantee+ Policy.
Click
here for details.
|
|
Distributor Data...
|
|
anti-Insulin receptor subunit alpha antibody, anti-INSR alpha antibody, anti-HIR A antibody, anti-IRS1 antibody, anti-IR antibody, anti-Insulin receptor antibody, anti-INSR antibody, anti-HIRS-1 antibody, anti-HHF5 antibody, anti-CD220 antigen antibody, anti-CD220 antibody, |
|
|
Insulin Receptor Antibody (MA-20) Summary: | | Gene: | INSR |
|---|
| Purity: | Affinity purified |
|---|
| Host: | Mouse |
|---|
| | Specificity: | This is specific for Insulin Receptor. |
|---|
| Publications: | Antibody has been mentioned in at least 13 Publications. |
|---|
| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional Insulin Receptor Products Insulin Receptor Antibody (MA-20) Details: | Clone: | MA-20 |
|---|
| Isotype: | IgG2b |
|---|
| Immunogen: | Highly purified insulin receptors (alpha-subunit) from human term placentas. |
|---|
| Localization: | Membrane; single pass type I membrane protein. |
|---|
Human and rat. Other species have not been tested. |
| Uses: | This antibody is useful for ELISA, immunoprecipitation, Western blot and competition assays. |
|---|
| Dilutions: | ELISA,
immunoprecipitation,
Western Blot,
Blocking/Neutralizing, |
|---|
| Unit Size: | 0.1 ml (also available: 0.025 ml (sample size)) |
|---|
| Concentration: | 1.2 mg/ml |
|---|
| Positive Controls: | 1 Positive Controls |
|---|
| Conjugated Antibodies: | 5 Conjugated Antibodies |
|---|
|
Packaging: | Storage: | Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles. |
|---|
| Buffer: | 10 mM phosphate buffer [pH 7.4], 100 mM NaCl. |
|---|
| Preservative: | 0.1% Sodium Azide |
|---|
| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
|---|
Background: The insulin receptor is functionally important for negative cooperativity, down-regulation, and beta-subunit tyrosine kinase activity. There are two recognized epitopes of the alpha-subunit. One epitope is close to the insulin-binding site and plays a role in insulin-induced negative cooperativity and down-regulation. The other site (recognized by the monoclonal MA-20 antibody) is at a distance from the insulin-binding site, where it only plays a role in down-regulation. Both epitopes appear to be independent of insulin receptor tyrosine kinase activity.
|
Reviews (0) Related Products (27) Exploring Insulin Receptor Working with Insulin Receptor
|
Jump to: Publications, Ask a Question
|
Working with Insulin Receptor
Insulin Receptor Antibody (MA-20) Images (0)
|
|
|
Publications (13)Insulin Receptor Antibody (MA-20) Product Specific:- Hawley DM, Maddux BA, Patel RG, Wong KY, Mamula PW, Firestone GL, Brunetti A, Verspohl E, Goldfine ID. Insulin receptor monoclonal antibodies that mimic insulin action without activating tyrosine kinase. J Biol Chem. 1989 Feb 15;264(5):2438-44. PubMed PMID: 2536710.
- Gherzi R, Russell DS, Taylor SI, Rosen OM. Reevaluation of the evidence that an antibody to the insulin receptor is insulinmimetic without activating the protein tyrosine kinase activity of the receptor. J Biol Chem. 1987 Dec 15;262(35):16900-5. PubMed PMID: 3680277.
more... - Costanzo BV, Trischitta V, Di Paola R, Spampinato D, Pizzuti A, Vigneri R, Frittitta L. The Q allele variant (GLN121) of membrane glycoprotein PC-1 interacts with the insulin receptor and inhibits insulin signaling more effectively than the common K allele variant (LYS121). Diabetes. 2001 Apr;50(4):831-6. PubMed PMID: 11289049.
- Milazzo G, Giorgino F, Damante G, Sung C, Stampfer MR, Vigneri R, Goldfine ID, Belfiore A. Insulin receptor expression and function in human breast cancer cell lines. Cancer Res. 1992 Jul 15;52(14):3924-30. PubMed PMID: 1617668.
- Maddux BA, Goldfine ID. Membrane glycoprotein PC-1 inhibition of insulin receptor function occurs via direct interaction with the receptor alpha-subunit. Diabetes. 2000 Jan;49(1):13-9. PubMed PMID: 10615944.
- Vella V, Pandini G, Sciacca L, Mineo R, Vigneri R, Pezzino V, Belfiore A. A novel autocrine loop involving IGF-II and the insulin receptor isoform-A stimulates growth of thyroid cancer. J Clin Endocrinol Metab. 2002 Jan;87(1):245-54. PubMed PMID: 11788654.
- Pandini G, Frasca F, Mineo R, Sciacca L, Vigneri R, Belfiore A. Insulin/insulin-like growth factor I hybrid receptors have different biological characteristics depending on the insulin receptor isoform involved. J Biol Chem. 2002 Oct 18;277(42):39684-95. Epub 2002 Jul 22. PubMed PMID: 12138094.
- Pandini G, Vigneri R, Costantino A, Frasca F, Ippolito A, Fujita-Yamaguchi Y, Siddle K, Goldfine ID, Belfiore A. Insulin and insulin-like growth factor-I (IGF-I) receptor overexpression in breast cancers leads to insulin/IGF-I hybrid receptor overexpression: evidence for a second mechanism of IGF-I signaling. Clin Cancer Res. 1999 Jul;5(7):1935-44. PubMed PMID: 10430101.
- Forsayeth JR, Caro JF, Sinha MK, Maddux BA, Goldfine ID. Monoclonal antibodies to the human insulin receptor that activate glucose transport but not insulin receptor kinase activity. Proc Natl Acad Sci U S A. 1987 May;84(10):3448-51. PubMed PMID: 3033648.
- Li, M, et al. Small molecule insulin receptor activators potentiate insulin action in insulin-resistant cells. Diabetes. 2001 Oct;50(10):2323-8. (ELISA, Western Blot, Rat)
- Federici, M, et al. Increased expression of insulin/insulin-like growth factor-I hybrid receptors in skeletal muscle of noninsulin-dependent diabetes mellitus subjects. J Clin Invest. 1996 Dec 15;98(12):2887-93. (Western Blot, Human)
- Frasca, F, et al. Insulin receptor isoform A, a newly recognized, high-affinity insulin-like growth factor II receptor in fetal and cancer cells. Mol Cell Biol. 1999 May;19(5):3278-88. (ELISA, Immunoprecipitation, Western Blot, Human)
- Forsayeth, J.R., et al. Effect of monoclonal antibodies on human insulin receptor autophosphorylation, negative cooperativity, and down-regulation. J Biol Chem. 1987 Mar 25;262(9):4134-40. (Immunoprecipitation, Compet. assay, human) http://www.jbc.org/content/262/9/4134.full.pdf
|
|
|
|
Ask a Scientist
Can't find an answer to your question about Insulin Receptor? Ask us by using live
chat or by submitting your questions below:
|
Jump to: Conjugated Antibodies, Positive Controls, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
|
Related ProductsConjugated Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
|---|
| Insulin Receptor Antibody (MA-20) [DyLight 488] | NB400-142G | Hu, Rt | B/N, ELISA, IP, WB | | | | | | Insulin Receptor Antibody (MA-20) [DyLight 550] | NB400-142R | Hu, Rt | B/N, ELISA, IP, WB | | | | | | Insulin Receptor Antibody (MA-20) [DyLight 650] | NB400-142C | Hu, Rt | B/N, ELISA, IP, WB | | | | | | Insulin Receptor Antibody (MA-20) [HRP] | NB400-142H | Hu, Rt | B/N, ELISA, IP, WB | | | | | | Insulin Receptor Antibody (MA-20) [Biotin] | NB400-142B | Hu, Rt | B/N, ELISA, IP, WB | | | | |
Positive Controls| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
|---|
Other Positive Controls- IM-9 lymphocytes and HEP-G2 cells
|
Lysates (1)Peptides and ProteinsPrimary Antibodies (15)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
|---|
| Insulin Receptor [p Tyr1361] Antibody | NBP1-51404 | Hu, Mu, Rt | IHC-P, WB | (2) | | | | | Insulin Receptor [p Tyr1185] Antibody (EP351(2)Y) | NB110-62109 | Hu | ICC, IP, WB | (1) | (3) | | | | Insulin Receptor Antibody (SPM258) | NBP1-74565 | Hu | ELISA, IHC-P, IP, WB | (1) | (1) | | | | Insulin Receptor Antibody | NBP1-47580 | Hu, Mu, Rt | IHC-P, WB | | | | | | Insulin Receptor Antibody | NB300-337 | Hu | WB | (1) | (1) | | | | ... and 10 more. See All » |
RNAi (1)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
|---|
| Mouse IgG Antibody [Biotin] | NB720-B | Mu | ELISA, IHC-P, WB | | | | | | Mouse IgG Antibody [HRP] | NB720-H | Mu | ELISA, ICC, IHC-P, WB | | | | | | Mouse IgG Antibody [Alkaline Phosphatase] | NB720-AP | Mu | ELISA, ICC, IHC-P, WB | | | | | | Mouse IgG Antibody [FITC] | NB720-F | Mu | ICC, IHC-P | | (2) | | | | Mouse IgG Antibody | NB120-7056 | Bv(-), Ch(-), Eq(-), Gp(-), Gt(-), Ha(-), Hu(-), Mu, Rb(-), Rt(-), Sh(-) | ELISA, IHC-P, IP, WB | | | | | | See All » |
|
Jump to: Novus Explorer, Research Areas
| Insulin Receptor in Context
Research Areas related to Insulin Receptor (7): |
|
Novus Explorer for Insulin Receptor gene
|
|
