I read about one of your other IL1 beta antibodies that primarily detects the mature 17 kDa protein, but also to some extent the pro-protein which is cleaved to the 17kDa product by the heating applied in the preparation of the samples for WB. Is this also the case for this antibody (NB120-10747) or does it only detect the "biologically" cleaved protein?

Name: IL-1 alpha/IL-1F1 Antibody (59015.111)
Brand: Novus Biologicals
SKU: NB120-10747
Price: 279 USD
Availability: Discontinued

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Excessive heating or prolonged heating of your samples can cause peptide bond cleavage and protein degradation. It is impossible to differentiate between the biologically cleaved and heat induced peptide bond cleavage. For detecting proforms of protein, following addition of SDS sample buffer, the sample should be heated to 85C for 3 minutes. The standard western blot protocol calls for boiling (95C) for 10 minutes however in this case decreasing the time and temperature will help detect the proform of the protein.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Immunogen	recombinant rat interleukin-1alpha, expressed in Escherichia coli.
Localization	Secreted
Specificity	IL1 alpha (59015.111)
Isotype	IgG1
Clonality	Monoclonal
Host	Mouse
Gene	IL1A
Purity	Protein G purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Dilutions	ELISA 1:100-1:2000 Western Blot 1-2 ug/ml
Application Notes	Capture ELISA: this antibody can be used as a capture antibody in a rat IL-1a ELISA with a biotinylated detection antibody. It is recommended to use plates coated with 100 mL/well of the capture antibody at a concentration of 1 mg/mL. Immunoblotting: a working concentration of 1-2 ug/mL is recommended using 50 ng/lane of rat IL-1a under either reducing or non-reducing conditions.

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	PBS and 5% Trehalose
Preservative	No Preservative
Purity	Protein G purified

Gene Symbol	IL1A
Entrez	24493(Rat)

IL-1 alpha/IL-1F1 Antibody (59015.111)

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