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MHC class II (I-A/I-E) Antibody (M5/114.15.2)

 (2 Publications)
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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-77508
    • Availability
      Product Discontinued

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MHC class II (I-A/I-E) Antibody (M5/114.15.2) Summary

Immunogen
Activated C57BL/6 mouse spleen cells
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Rat
Gene
H2-Ea
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one million cells in 100 microliter volume. Immunohistochemistry-frozen sections. It is recommended that the reagent be titrated for optimal performance for each application.
Publications
Read Publications using
NB100-77508 in the following applications:

Reactivity Notes

Mouse, Reacts with a shared determinant on I-Ab,d,q, and I-Ed,k alloantigens..

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MHC class II (I-A/I-E) Antibody (M5/114.15.2)

  • E-alpha-f;H-2 class II histocompatibility antigen, E-K alpha chain;H-2 class II histocompatibility antigen, E-U alpha chain;H-2E;H2-Ea;H-2Ea;H2-Ea-ps;I;Ia;Ia3;Ia-3;I-E;I-Ealpha;MHC-H2-Ea
  • H2-Ea
  • MHC class II (I-A/I-E)
  • MHC class II IA/IE

Background

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The M5/114.15.2 antibody cross-reacts with the H-2p,r haplotypes. The M5/114.15.2 antibody has been reported to block antigen presentation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MHC class II
(I-A/I-E) Antibody (M ...
NB100-77508
MHC class II (I-A/I-E) Antibody (M5/114.15.2)
Species: Mu
Applications: Flow, ICC/IF, IHC, IP

Publications for MHC class II (I-A/I-E) Antibody (NB100-77508)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: FLOW.


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FLOW
(1)
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Showing Publications 1 - 2 of 2.
Publications using NB100-77508 Applications Species
Fabrik I, Link M, Hartlova A et al. Application of SILAC Labeling to Primary Bone Marrow-Derived Dendritic Cells Reveals Extensive GM-CSF-Dependent Arginine Metabolism J Proteome Res (FLOW, Mouse)

Details:
This publication used the Biotin conjugated form of this antibody (Cat# NB100-77509). 		FLOW Mouse
Fabrik I, Link M, Hartlova A et al. Application of SILAC Labeling to Primary Bone Marrow-Derived Dendritic Cells Reveals Extensive GM-CSF-Dependent Arginine Metabolism J Proteome Res 2013-12-07 [PMID: 24308431]

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Bioinformatics

Gene Symbol H2-Ea