This antibody (clone UT-1) reacts with CD95 (Fas/APO-1), a 46 kDa glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells. This antibody does excellently induce Fas mediated apoptosis, similarly as CH11 antibody.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
Type I membrane protein (isoform 1); Secreted (isoforms 2 to 6).
Species Reactivity:
Cross-reacts with Human.Not yet tested in other species.
Applications:
Uses:
Flow Cytometry Use at an assay dependent dilution. FuncS: Use at an assay dependent dilution. The antibody induces apoptosis of CD95 expressing cell lines.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:
PBS. pH 7.4
Preservative:
15mM Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
CD95, also known as FAS or APO1, is a 36 kDa cell surface type I membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS PAGE. CD95 is a member of the TNF receptor family, which includes TNFR1, TNFR2, CD27, CD30 and CD40. Binding of CD95 Ligand to CD95 or crosslinking of CD95 by anti CD95 monoclonal antibodies leads to apoptosis of CD95 expressing cells. CD95 belongs to a subgroup of family members that have a death domain (DD) which contains 70 amino acids near the carboxyl terminal region of the molecule. The binding of adaptor molecules to this DD is responsible for transmitting the death signal for apoptosis. Stimulation of CD95 results in aggregation of its DD, leading to the recruitment of FADD and caspase 8 that together with the receptor form the death inducing signaling complex (DISC). CD95/CD95L is involved in the peripheral deletion of activated mature T cells at the end of the immune response and defects in this pathway predispose to autoimmune disorders. CD95 is also involved in killing of targets such as virus infected cells or cancer cells and killing of inflammatory cells at immune privileged sites.
Flow cytometry / FACS analysis: Fas Antibody (UT-1) [NB120-11881] - Flow cytometry / FACS analysis: [NB120-11881] - Induction of apoptosis in various Hematopoetic cell lines by use of anti-Fas antibodies. Suspensions of used cell lines were incubated with soluble (or without as control) anti-Fas antibodies. Induction of apoptosis was measured by Apoptosis Assay Kit - FITC. Histograms: Red (full) = untreated control cells; Black = standard apoptosis-inducing anti-Fas antibody; Green = anti-Fas (UT-1).