AKT1 Antibody (NB100-79891)
AKT1 [p Ser473] Antibody (EP2109Y)
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Anti-RAC-PK-alpha antibody, anti-Protein kinase B antibody, anti-PKB antibody, Anti-C-AKT antibody, |
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AKT1 [p Ser473] Antibody (EP2109Y) Summary: | | Gene: | AKT1 |
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| Purity: | IgG purified |
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| Host: | Rabbit |
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| | Specificity: | The antibody detects Akt1 phosphorylated at Serine 473. |
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| Publications: | Antibody has at least 4 Background References. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional AKT1 Products AKT1 [p Ser473] Antibody (EP2109Y) Details: | Clone: | EP2109Y |
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| Isotype: | IgG |
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| Immunogen: | A phospho specific peptide corresponding to residues surrounding Ser473 of human Akt1 was used as immunogen. |
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| Modification: | p Ser473 |
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Reacts in Human and Mouse. Does not react in Rat. |
| Uses: | This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry. In Western blot this antibody detects a band at approximately 60kDa. |
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| Dilutions: | Western Blot 1:1000-10000, Immunohistochemistry-Paraffin 1:100-250, Immunocytochemistry 1:100-250 |
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| TMW: | 56 kDa |
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| Unit Size: | 0.1 ml |
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| Concentration: | Please see the vial label for concentration. |
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Packaging: | Storage: | Store at -20 °C. Avoid freeze-thaw cycles. |
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| Buffer: | 50mM Tris-Glycine [pH 7.4] 0.15M NaCl, 40% Glycerol and 0.05% BSA |
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| Preservative: | 0.01% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: Akt1/PKBa is a Ser/Thr protein kinase whose kinase activity plays a key role in various cellular functions, including apoptosis, glycogen synthesis, and cell growth . Akt1/PKBa is activated by various growth and survival factors (1). Phosphorylation on Thr308, Ser473 and Tyr474 is required for full activity. Akt1/PKBa inhibits apoptosis by phosphorylating and inactivating several targets, including caspase-9 (2). Akt1/PKBa regulates glycogen synthesis by phosphorylating and inactivating glycogen synthase kinase-3a and b (3,4). Three mammalian Akt1/PKBa isoforms have been identified.
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Images (3) Reviews (0) Related Products (105) Exploring AKT1 Working with AKT1
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Jump to: Publications, Images, Ask a Question
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Working with AKT1
AKT1 [p Ser473] Antibody (EP2109Y) Images (3)-Western Blot-NB100-79891-img0006.jpg "Western Blot: AKT1 [phospho Ser473] Antibody (EP2109Y) [NB100-79891] - Western blot analysis on 3T3 cell lysates using NB100-79891, 1:10,000 dilution. Cells were either (A) untreated or (B) treated with PDGF.") Western Blot: AKT1 [phospho Ser473] Antibody (EP2109Y) [NB100-79891] - Western blot analysis on 3T3 cell lysates using NB100-79891, 1:10,000 dilution. Cells were either (A) untreated or (B) treated with PDGF. | -Immunohistochemistry-NB100-79891-img0004.jpg "Immunohistochemistry: AKT1 [phospho Ser473] Antibody (EP2109Y) [NB100-79891] - Immunohistochemical analysis of Phosphatase- treated cervical carcinoma using NB100-79891.") Immunohistochemistry: AKT1 [phospho Ser473] Antibody (EP2109Y) [NB100-79891] - Immunohistochemical analysis of Phosphatase- treated cervical carcinoma using NB100-79891. | -Immunohistochemistry-NB100-79891-img0003.jpg "Immunohistochemistry: AKT1 [phospho Ser473] Antibody (EP2109Y) [NB100-79891] - Immunohistochemical analysis of untreated cervical carcinoma using NB100-79891.") Immunohistochemistry: AKT1 [phospho Ser473] Antibody (EP2109Y) [NB100-79891] - Immunohistochemical analysis of untreated cervical carcinoma using NB100-79891. |
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Publications (4)AKT1 [p Ser473] Antibody (EP2109Y) Background References:- Cross, D.A., et al. Nature 378: 785-789 (1995).
- Hajduch, E., et al. FEBS Lett. 492: 199-203 (2001).
more... - Cardone, M.H., et al. Science 282: 1318-1321 (1998).
- Burgering, B.M. P.J. Coffer.Nature 376: 599-602 (1995).
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Jump to: Antibody Pairs, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsAntibody Pairs (6)Lysates (2)Peptides and ProteinsPrimary Antibodies (85)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| AKT1 [p Ser473] Antibody | NB600-590 | Hu | ELISA, ICC, IF, IHC-P, WB | (6) | (1) | | | | AKT1 Antibody (2E11) | H00000207-M03 | Hu, Mu, Rt | ELISA, IF, IHC-P, WB | (7) | | | | | AKT1 Antibody | NB600-467 | Hu | ELISA, IF, IHC-P, WB | (4) | (1) | | | | AKT1 Antibody (Y89) | NB110-55448 | Hu, Mu, Rt | FACS, ICC, IHC-P, IP, WB | (2) | (1) | | | | AKT1 Antibody (6F11) | H00000207-M09 | Hu | ELISA, IF, WB | (6) | | | | | ... and 80 more. See All » |
RNAi (5)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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