PD-1 Antibody (J116) - BSA Free

Flow Cytometry: PD-1 Antibody (J116) [NBP1-43107] - Staining of human PD-1 transfected cells with anti-human Anti-Human CD279 (PD-1) FITC (left)or PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

• Reactivity: Hu
• Applications: WB, Flow, IHC, IP
• Clone: J116
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

PD-1 Antibody (J116) - BSA Free Summary

• Description: Novus Biologicals Mouse PD-1 Antibody (J116) - BSA Free (NBP1-43107) is a monoclonal antibody validated for use in IHC, WB, Flow and IP. Anti-PD-1 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Clone J116 was used by HLDA to establish CD designation.
• Immunogen: The immunogen for this antibody was PD1.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDCD1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: The J116 antibody has been tested by flow cytometric analysis of human PD-1 transfected cells. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for PD-1 Antibody (J116) - BSA Free

• CD279 antigen
• CD279
• hPD-1
• PD1
• PD-1
• PD1hPD-l
• PDCD1
• programmed cell death 1
• programmed cell death protein 1
• Protein PD-1
• SLEB2

Background

The J116 monoclonal antibody reacts with the human PD-1 (programmed death-1), a 55 kDa member of the immunoglobulin superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. PD-1 is expressed predominantly on activated T and B lymphocytes. Two novel members of the B7 family have been identified as the PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Binding of the J116 monoclonal antibody inhibits PD-1 signal transduction, however, it does not block binding of the ligand PD-L1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PD-1 Antibody (NBP1-43107)(4)

Publications using NBP1-43107

• Mohr M, Damas N, Gudmand-H�yer J et al. The CRISPR-Cas12a Platform for Accurate Genome Editing, Gene Disruption, and Efficient Transgene Integration in Human Immune Cells ACS synthetic biology 2023-02-17 [PMID: 36750230] (Flow Cytometry, Human)
• H. B. Deepak, Sabina Evan Prince, Pratima Deshpande Effect of baricitinib in regulating programmed death 1 and ligand programmed cell death ligand 1 through JAK/STAT pathway in psoriasis Indian Journal of Pharmacology 2022-01-01 [PMID: 35848689]
• Peled M, Strazza M, Azoulay-Alfaguter I, Mor A. Analysis of Programmed Death-1 in Patients with Psoriatic Arthritis Inflammation. 2015-02-10 [PMID: 25663558] (FLOW, Human)
• Azoulay-Alfaguter I, Strazza M, Pedoeem A, Mor A. The coreceptor programmed death-1 inhibits T-cell adhesion by regulating Rap1. J Allergy Clin. Immunol. 2014-09-17 [PMID: 25240786]

Bioinformatics

• Gene Symbol: PDCD1