Integrin alpha E/CD103 Antibody (B-Ly7) - BSA Free

Flow Cytometry: Integrin alpha E/CD103 Antibody (B-Ly7) [NBP1-43370] - Analysis using the FITC conjugate of NBP1-43370. Staining of 3-day PHA-stimulated normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control FITC (blue histogram) or Anti-Human CD103 (Integrin alpha E) FITC (purple histogram).

Flow Cytometry: Integrin alpha E/CD103 Antibody (B-Ly7) [NBP1-43370] - CD103/Integrin alpha E Antibody (B-Ly7) Staining of 3-day PHA-stimulated normal human peripheral blood cells with 0.25 ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or 0.25 ug of Anti-Human CD103 (Integrin alpha E) Purified (filled histogram) followed by Anti-Mouse IgG FITC .

• Reactivity: Hu
• Applications: Flow, IHC
• Clone: B-Ly7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

Integrin alpha E/CD103 Antibody (B-Ly7) - BSA Free Summary

• Additional Information: Clone B-Ly7 was used by HLDA to establish CD designation.
• Immunogen: The immunogen for this antibody was CD103/Integrin alpha E.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGAE
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
• Application Notes: The B-Ly7 antibody has been tested by flow cytometric analysis of 2-3 day PHA-activated human peripheral blood leukocytes. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for Integrin alpha E/CD103 Antibody (B-Ly7) - BSA Free

• antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide
• CD103 antigen
• CD103
• HML-1 antigen
• HUMINAE
• Integrin alpha E
• integrin alpha-E
• Integrin alpha-IEL
• integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alphapolypeptide)
• ITGAE
• MGC141996
• Mucosal lymphocyte 1 antigen

Background

The B-Ly7 monoclonal antibody reacts with human CD103/Integrin alpha E, the alphaE integrin. CD103/Integrin alpha E non-covalently associates with integrin beta7. CD103/Integrin alpha E is expressed mainly on intraepithelial lymphocytes and a small subset of peripheral lymphocytes. CD103/Integrin alpha E is also expressed by hairy cell leukemia (HCL) and by some chronic B cell lymphocytic leukemias. In vitro stimulation of human T cells with mitogens induces upregulation of CD103/Integrin alpha E. Epithelial cell antigen, E-cadherin, binds to CD103/Integrin alpha E and mediates homing of lymphocytes to the intestinal epithelium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGAE