ABP antibody

Rabbit Polyclonal anti-ABP

Catalog Number: NB100-56417	Price: $295.00

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Background:

The gene encodes an EF hand-containing small calcium binding protein. It is an integral subunit component of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Kv4 potassium channels regulate action potentials in neurons and cardiac myocytes. The coexpression of ABP with pore-forming Kv4 alpha subunits causes changes in the gating and amplitude of Kv4 currents, thus profoundly affect the intracellular trafficking and molecular properties of Kv4.2 alpha subunits. It also cause a dramatic redistribution of Kv4.2, releasing intrinsic endoplasmic reticulum retention and allowing for trafficking to the cell surface. It may regulate A-type currents, and hence neuronal excitability, in response to changes in intracellular calcium. It maps to 5q35.1 region of human chromosome. Alternative splicing results in multiple transcript variant encoding different isoforms. Isoform 1 and isoform 2 are expressed in brain and kidney.

Alternate Names:

anti-VABP antibody, anti-Vesicle APC-Binding Protein antibody, anti-KCHIP2 antibody

Host:

Rabbit

Research Areas:

Vision

Immunogen:

Amino acids 11-28 (LQTKQRRPSKDKIEDELE) of human ABP protein were used as the immunogen.

Isotype:

IgG

Specificity:

The NCBI accession number for ABP is NP-055407. The amino acid sequence used as immunogen is 100% homologous in human, mouse, rat, pig and bovine.

Species Reactivity:

Reacts with Human, mouse, rat, porcine and bovine.
Other species have not been tested.

Homology:

Mouse 100%
Rat 100%
Pig 100%
Bovine 100%

Uses:

Western Blot
Other applications have not been tested.

Dilutions:

Suggested working dilutions *
Western Blot 0.5-2 ug/ml
* Investigator should determine optimal working dilutions.

Positive Controls:

human brain or heart
* Human Brain Protein NB820-59177
* Human Heart Protein NB820-59217

Packaging:

0.1 mg protein G purified Rabbit antisera.

Buffer:

PBS containing 0.2% gelatin

Preservative:

0.05% sodium azide

Storage:

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Limitations:

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 6 months from date of receipt.

General References:

1. Lin,Y.L., Chen,C.Y., Cheng,C.P. and Chang,L.S. Protein-protein interactions of KChIP proteins and Kv4.2. Biochem. Biophys. Res. Commun. 321 (3), 606-610 (2004).
2. Nakamura,T.Y., Nandi,S., Pountney,D.J., Artman,M., Rudy,B. and Coetzee,W.A. Different effects of the Ca(2+)-binding protein, KChIP1, on two Kv4 subfamily members, Kv4.1 and Kv4.2. FEBS Lett. 499 (3), 205-209 (2001).
3. W. Frank An, Mark R. Bowlby, Maria Betty, Jie Cao, Huai-Ping Ling, Grace Mendoza, Joseph W. Hinson, Karen I. Mattsson, Brian W. Strassle, James S. Trimmer and Kenneth J. Rhodes. Modulation of A-type potassium channels by a family of calcium sensors; Nature 403, 553-556 (3 February 2000).

Gene Id:

6462

Gene System:

SHBG

Western blot analysis of ABP in (A) human brain and (B) human heart cell lysate using NB100-56417 at 1 ug/ml.

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