ACT1 antibody

Rabbit Polyclonal anti-ACT1

Catalog Number: NB100-56317	Price: $325.00

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Background:

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (3). External stimuli such as tumor necrosis factor or other cytokines, such as IL-1 results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes (2). Recently, Li et al. (1) have isolated a cDNA coding for a protein named Act1 (NF- B activator 1). Act1 cDNA codes for a protein containing 574 aa with a predicated molecular mass of 60 kDa. Act1 protein contains a helix-loop-helix domain in its N-terminal portion and a coiled-coil structure at the C terminus. Act1 lacks any catalytic domain, however, it activates IKK through the helix-loop-helix domain. The activation of NF- B by Act1 appears to be through the NIK-IKK complex, because a NIK dominant-negative mutant protein inhibited Act1-induced NF- B activation. IL-1 responsive pathway is also necessary for Act1 function. Act1 mRNA is expressed in a variety of tissues, with high level in thymus, kidney and placenta; moderate level in heart, skeletal muscle, colon, liver, lung and small intestine; and at a very low level in brain, spleen, and peripheral blood leukocytes.

Host:

Rabbit

Immunogen:

A mixture of synthetic peptides corresponding to amino acids 341-351 (CSFPGLPRHQDQPHHQ), 91-104 (CLRTQVLEDSEDSF) and 562-574 (PRGPLPTLQVVPLC) of human ACT1 was used as immunogen, GenBank no. gi|10242290|gb|AAG15367.1|AF274303_1 .

Isotype:

IgG

Specificity:

In MCF-7, an approx. 60 kDa band is observed.

Species Reactivity:

Cross reacts with Human.
Other species have not been tested.

Uses:

Western Blot
Other applications have not been tested.

Dilutions:

Suggested working dilutions *
Western Blot 1-2 ug/ml
* Investigator should determine optimal working dilutions.

Positive Controls:

MCF-7
* MCF-7 Whole Cell Lysate NB820-59465

Packaging:

0.1 mg protein G purified Rabbit antisera.

Buffer:

PBS

Preservative:

0.02% Sodium Azide

Storage:

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Limitations:

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 6 months from date of receipt.

General References:

1. Verma, I.M., Stevenson, J.K., Schwarz, EM., Van Antwerp, D. & Miyamoto, S. Genes. Dev. 9: 2723-2735 (1995).
2. Verma, I. And Stevenson, J.K. Proc. Nat. Acad. Sci. USA 94: 11758 (1997).
3. Li X, Commane M, Nie H, Hua X, Chatterjee-Kishore M, Wald D, Haag M, and Stark GR. Proc Natl Acad Sci U S A 97(19):10489-10493 (2000).

Gene Id:

10758

Gene System:

TRAF3IP2

Western blot analysis of 10 ug of total cell lysate from MCF-7, a human breast cancer cell line, with NB100-56317 at 1 ug/ml dilution.

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