Rat Lung Whole Tissue Lysate (Adult Whole Normal) Summary
| Localization |
Lung |
| Specificity |
Rat lung tissue lysate (non-denatured) |
Applications/Dilutions
| Dilutions |
- Immunoprecipitation
- Western Blot
|
| Application Notes |
Western Blot, Immunoprecipitation |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles. |
| Buffer |
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. |
| Concentration |
5 mg/ml |
Lysate Details for Array
| Type |
Tissue |
| Life Stage |
Adult |
| Tissue |
Respiratory |
| Protein State |
Native |
| Tissue Condition |
Normal |
| Subcellular Fraction |
Whole |
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are
guaranteed for 6 months from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for Lung Lysate (L037W2). (Showing 1 - 1 of 1 FAQ).
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Will you please explain the difference between the two products for me: NBP2-25014 and L037W2. In general what is the difference between Rat Lung Tissue Lysate and Rat Lung Whole Tissue Lysate? Also, I see that there are options for denatured and non-denatured Rat Lung Whole Tissue Lysates - is the first product (Rat Lung Tissue Lysate) denatured?
- These are both whole cell lsysates but there are a few differences between these two products. For one, NBP2-25014 is a Novus Biologicals product. L037W2 is produced by a Taiwanese company called Abnova and we distribute for them. More importantly, NBP2-25014 comes in 1 x SDS sample buffer: 60 mM Tris-Hcl (pH 6.8) 10 mM EDTA 2 % SDS 0.03% Bromophenol Blue 10% glycerol 0.3% Beta-mercaptoethanol as a sample buffer. The 0.3% BME means that this product is reduced and denatured. L037W2 comes in Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. So it is not reduced or denatured.
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